Objective      To suppress the gene expression of HDAC1 in human esophageal cancer cell line EC109 and to observe its impact on cell growth.       Methods      Synthesized oligonucleotide was cloned into a lentiviral vector pGCSIL-PUR digested with AgeⅠ and EcoRⅠ. The recombinant vector was further screened by PCR and verified by sequencing

and was transfected into package cells 293T with helper vectors. Recombinant lentivirus and control were extracted from cell culture and were used to infect EC109 cells. Individual cell clone was picked up and gene expression of HDAC1 was determined by real-time PCR and Western blotting. Cell growth and cell cycle distribution were detected by CCK-8 and FACS

respectively.       Results      Recombinant vector was successfully constructed

and sufficient lentivirus was obtained from package cells. The recombinant lentivirus can efficiently infect EC109 cells

and two clones were picked up from the infected EC109 cells. The results of real-time PCR and Western blotting showed HDAC1 gene expression was efficiently suppressed in EC109 cells

of which cell growth and cell cycle distribution were significantly altered.       Conclusion      The RNAi technology based on lentivirus can efficiently suppress the gene expression of HDAC1 in EC109 cells

and lead to cell growth inhibition.