Expression of snail in human small-cell lung cancer cell line H446 and its role in cisplatin resistance

况里杉; 周向东

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Expression of snail in human small-cell lung cancer cell line H446 and its role in cisplatin resistance

更新时间：2026-01-09

- Expression of snail in human small-cell lung cancer cell line H446 and its role in cisplatin resistance
- Journal of Army Medical University Vol. 35, Issue 12, Pages: 1197-1199(2013)
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  CLC： R394.2;R734.2;R979.11
- Published：2013
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况里杉,周向东.人小细胞肺癌细胞H446 Snail基因表达及其在顺铂耐药中的作用[J].陆军军医大学学报(原第三军医大学学报),2013,35(12):1197-1199.

　Kuang Lishan,Zhou Xiangdong.Expression of snail in human small-cell lung cancer cell line H446 and its role in cisplatin resistance[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(12):1197-1199.
况里杉,周向东.人小细胞肺癌细胞H446 Snail基因表达及其在顺铂耐药中的作用[J].陆军军医大学学报(原第三军医大学学报),2013,35(12):1197-1199. DOI：

　Kuang Lishan,Zhou Xiangdong.Expression of snail in human small-cell lung cancer cell line H446 and its role in cisplatin resistance[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(12):1197-1199. DOI：

摘要

目的观察锌指转录因子snail在耐顺铂（cisplatin

CDDP）的人小细胞肺癌(small cell lung cancer

SCLC)H446/CDDP细胞中的表达情况，探讨snail表达在人SCLC顺铂耐药中的作用。方法培养H446细胞系和H446/CDDP细胞系，采用MTT法检测H446/CDDP的耐药指数；分别提取人SCLC顺铂耐药细胞H446/CDDP及其亲代细胞H446的mRNA及总蛋白，应用sybgreen实时荧光RT-PCR、Western blot方法检测snail mRNA及蛋白表达水平。结果 H446/CDDP细胞snail蛋白及mRNA表达水平较H446细胞显著性升高(P0.05)。结论 SCLC的顺铂耐药性可能与snail表达水平增高有关。

Abstract

Objective To observe the expression of zinc finger transcription factor snail in human cisplatin (CDDP)-resistant small-cell lung cancer (SCLC) cell line H446/CDDP

and to study the role of snail in cisplatin resistance of human SCLC. Methods H446 and H446/CDDP cell lines were cultured

and cisplatin resistance index was measured by MTT assay. The mRNA and protein expression levels of snail in the H446 and H446/CDDP cells were detected by real-time RT-PCR and Western blotting. Results The mRNA and protein expression levels of snail in the H446/CDDP cells were significantly higher than those in the H446 cells(P0.05). Conclusion The CDDP resistance of human SCLC may be related to the high expression level of snail.

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