EMILIN3 stimulates human mesenchymal stem cells proliferation and differentiation into cartilage cells in vitro

秦川; 张峡; 王直兵; 郭星宇; 张瑗; 郝勇

您当前的位置：

首页 >

文章列表页 >

EMILIN3 stimulates human mesenchymal stem cells proliferation and differentiation into cartilage cells in vitro

更新时间：2026-01-09

- EMILIN3 stimulates human mesenchymal stem cells proliferation and differentiation into cartilage cells in vitro
- Journal of Army Medical University Vol. 35, Issue 11, Pages: 1053-1057(2013)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R322.71;R329.26;R394.2
- Published：2013
- 稿件说明：
移动端阅览
秦川,张峡,王直兵,郭星宇,张瑗,郝勇.EMILIN3基因促进人骨髓间充质干细胞增殖及向软骨细胞分化[J].陆军军医大学学报(原第三军医大学学报),2013,35(11):1053-1057.

　Qin Chuan,Zhang Xia,Wang Zhibin,Guo Xingyu,Zhang Yuan,Hao Yong.EMILIN3 stimulates human mesenchymal stem cells proliferation and differentiation into cartilage cells in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(11):1053-1057.
秦川,张峡,王直兵,郭星宇,张瑗,郝勇.EMILIN3基因促进人骨髓间充质干细胞增殖及向软骨细胞分化[J].陆军军医大学学报(原第三军医大学学报),2013,35(11):1053-1057. DOI：

　Qin Chuan,Zhang Xia,Wang Zhibin,Guo Xingyu,Zhang Yuan,Hao Yong.EMILIN3 stimulates human mesenchymal stem cells proliferation and differentiation into cartilage cells in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(11):1053-1057. DOI：

摘要

目的探讨EMILIN3基因对人骨髓间充质干细胞(human marrow mesenchymal stem cells

hMSCs)增殖及向软骨细胞分化的影响。方法构建EMILIN3腺病毒表达载体，细胞分成hMSCs、hMSCs+对照腺病毒、hMSCs+EMILIN3腺病毒3组，MTT法检测细胞的增殖情况，Real-time PCR法检测上述细胞向软骨细胞诱导后CollagenⅡ、Aggrecan、SOX9 mRNA的表达。结果 hMSCs+EMILIN3腺病毒组细胞增殖能力明显快于hMSCs+对照腺病毒组和hMSCs组(

0.05)，hMSCs+对照腺病毒组与hMSCs组增殖能力相比有所降低，但差异无统计学意义(

0.05)。hMSCs+EMILIN3腺病毒组细胞中CollagenⅡ、Aggrecan、SOX9 mRNA的相对表达量与hMSCs+对照腺病毒组、hMSCs组相比明显增加（

0.05，

0.01)。hMSCs+对照病毒组与hMSCs组相比有所增加，但没有明显的趋势（

0.05）。结论 EMILIN3促进hMSCs增殖，并且可以增加hMSCs向软骨细胞诱导分化。

Abstract

Objective To determine the effect of EMILIN3 gene on the proliferation of human marrow mesenchymal stem cells (hMSCs) and the differentiation into cartilage cells. Methods The adenovirus plasmid of EMILIN3 was constructed. The cells were transfected with the plasmid or the blank vector

and the cells without transfection served as control. The proliferation of these cells was detected by MTT assay. The expression of CollagenⅡ

Aggrecan and SOX9 at mRNA level was also detected with real-

time PCR. Results The proliferation was significantly increased in the hMSCs infected by the adenovirus plasmid of EMILIN3 than the cells with blank vector infection and control cells (

0.05). But there was no difference in the proliferation between the 2 later groups of cells (

0.05). The expression of CollagenⅡ

Aggrecan and SOX9 was increased by 2.5

2.7 and 2.1 times respectively in the hMSCs infected by the adenovirus with EMILIN3 than that in the hMSCs infected by the control adenovirus (

0.05). Compared with the hMSCs

there was no difference in the expression of above genes in the hMSCs infected by the blank vector. Conclusion EMILIN3 transfection enhances hMSCs proliferation and stimulate the cells to differentiate into cartilage cells.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Recombinant adenovirus containing human TFF3 gene regulates migration in human biliary epithelial cells

Wnt7a gene stimulates mesenchymal stem cell proliferation and differentiation into neuron-like cells

Effect of SDF-1/HOXB4 fusion gene-modified mesenchymal stem cells on amplification and stemness maintenance of hematopoietic stem cells in vitro

Expression of deltex-1 in rat bone marrow mesenchymal stem cells by adenovirus vector

Normal human liver cell line L02 appears immunological inhibition after by CTLA4Ig gene modification

Related Author

郑树国

程哲

田驹

阴洪

王开见

蒋涛

任先军

周长立

Related Institution

No data

⁰