Objective      To assess the role of silent mating type information regulation 2 homolog 1/uncoupling protein 2 (SIRT1/UCP2) signaling pathway in the inhibition of oxidative damage in vascular endothelial cells by resveratrol.        Methods      Human umbilical vein endothelial cells (HUVECs) were isolated and primarily cultured. The obtained cells were treated by tert-butyl hydrope

roxide (t-BHP) at the concentrations of 20

30

40

50

60

70

80

90 and 100 μmol/L respectively for 24 h

and CCK-8 assay was used for cell viability to determine the IC

50

value. The intracellular level of reactive oxygen species (ROS) was observed by fluorospectrophotometry. The expression of SIRT1 and UCP2 at mRNA level was determined by qRT-PCR assay. The protein expression of SIRT1

UCP2 and Caspase-3 were determined by Western blot assay.       Results      Cell viability was significantly inhibited by t-BHP treatment （

P

0.05）and the IC

50

 value was identified as 80 μmol/L. Pretreatment with resveratrol at the doses of 0.1

1

and 10 μmol/L significantly inhibited the decrease of cell viability （P0.05） and the increase of intracellular ROS level induced by t-BHP. Furthermore

the mRNA and protein expression of SIRT1 were up-regulated by resveratrol pretreatment

and that of UCP2 was down-regulated

yet which was abolished by niacinamide pretreatment.       Conclusion      Resveratrol may suppress the expression of UCP2 by activating SIRT1

and attenuate the increase of intracellular ROS level induced by t-BHP

and thus

inhibit t-BHP-induced endothelial injury.