In vitro antitumor efficacy of novel docetaxel-loaded polyethylene glycol-modified poly(lactic acid) nanoparticles

赵俪梅; 张寅星; 陈振波; 梁志清

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In vitro antitumor efficacy of novel docetaxel-loaded polyethylene glycol-modified poly(lactic acid) nanoparticles

更新时间：2026-01-09

- In vitro antitumor efficacy of novel docetaxel-loaded polyethylene glycol-modified poly(lactic acid) nanoparticles
- Journal of Army Medical University Vol. 35, Issue 11, Pages: 1048-1052(2013)
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  CLC： R737.31;R944.9;R979.1
- Published：2013
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赵俪梅,张寅星,陈振波,梁志清.新型生物素接枝改性聚乳酸-多烯紫杉醇纳米粒体外抗肿瘤研究[J].陆军军医大学学报(原第三军医大学学报),2013,35(11):1048-1052.

　Zhao Limei,Zhang Yinxing,Chen Zhenbo,Liang Zhiqing.In vitro antitumor efficacy of novel docetaxel-loaded polyethylene glycol-modified poly(lactic acid) nanoparticles[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(11):1048-1052.
赵俪梅,张寅星,陈振波,梁志清.新型生物素接枝改性聚乳酸-多烯紫杉醇纳米粒体外抗肿瘤研究[J].陆军军医大学学报(原第三军医大学学报),2013,35(11):1048-1052. DOI：

　Zhao Limei,Zhang Yinxing,Chen Zhenbo,Liang Zhiqing.In vitro antitumor efficacy of novel docetaxel-loaded polyethylene glycol-modified poly(lactic acid) nanoparticles[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(11):1048-1052. DOI：

摘要

目的以人卵巢癌细胞SKOV3为模型

研究负载多烯紫杉醇（TXT）的新型生物素接枝改性聚乳酸纳米粒（TXT-BPLA NPs）的体外抗肿瘤作用。方法以不同剂量TXT-BPLA NPs（1、10、100、1 000、10 000 nmol/L）处理SKOV3细胞不同时间（12、24、48、72、96、120 h）后用CCK-8检测细胞增殖抑制率，流式细胞仪检测细胞凋亡率，Western blot检测caspase-3表达。结果在10～10 000 nmol/L浓度范围内，相同时间点下，TXT和TXT-BPLA NPs对SKOV3细胞生长的抑制率均呈现出剂量依赖性。在相同浓度下，TXT组在24 h和48 h对SKOV3细胞的增殖抑制率、凋亡率及caspase-3的表达均明显高于TXT-BPLA NPs组（

0.05）；72 h后，TXT-BPLA NPs组对SKOV3细胞的增殖抑制率、凋亡率及caspase-3的表达均明显高于TXT组（

0.05）。结论与TXT相比，TXT-BPLA NPs能够更加持续地抑制SKOV3细胞的增殖，并促进caspase-3介导的细胞凋亡，具有更强的持续性抗肿瘤作用。

Abstract

Objective To determine the antitumor effects of the novel docetaxel (TXT)-loaded biotinylated poly (lactic acid) nanoparticles (TXT-BPLA NPs) on human ovarian cancer cell line SKOV3. Methods SKOV3 cells were cultured with TXT-BPLA NPs at different concentrations (1

100

1 000 and 10 000 nmol/L) and durations (12

96 and 120 h) respectively. Then proliferation and apoptosis of SKOV3 cells were determined by CCK-8 assay and flow cytometry

respectively. The apoptosis-related prot

ein caspase-3 was measured by Western blotting. Results Both TXT and TXT-BPLA NPs resulted in inhibited proliferation in SKOV3 cells in a dose-dependent manner when the concentration was given at 10 to 10 000 nmol/L within same duration. Under the same dose

TXT-treated SKOV3 cells for 24 and 48 h had obviously higher proliferative rate

apoptotic rate and caspase-3 expression than the cells treated by TXT-BPLA NPs (

0.05)

while these phenomena was reversed when the treatment time was prolonged to 72 h (

0.05). Conclusion Comparing with TXT

TXT-BPLA NPs could more persistently inhibit the proliferation of SKOV3 cells and promote caspase-3-mediated apoptosis. Thus

TXT-BPLA NPs

as a new nanometer drug

has a special application value for the therapy of ovarian cancer.

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