Sequencing and bioinformatic analysis of genome of Acinetobacter baumannii bacteriophage AB3

张劼; 刘茜; 甘丹

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Sequencing and bioinformatic analysis of genome of Acinetobacter baumannii bacteriophage AB3

更新时间：2026-01-09

- Sequencing and bioinformatic analysis of genome of Acinetobacter baumannii bacteriophage AB3
- Journal of Army Medical University Vol. 35, Issue 15, Pages: 1562-1565(2013)
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  CLC： Q939.48;Q755
- Published：2013
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张劼,刘茜,甘丹.鲍曼不动杆菌噬菌体AB3的全基因组测序及生物信息学分析[J].陆军军医大学学报(原第三军医大学学报),2013,35(15):1562-1565.

　Zhang Jie,Liu Xi,Gan Dan.Sequencing and bioinformatic analysis of genome of Acinetobacter baumannii bacteriophage AB3[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(15):1562-1565.
张劼,刘茜,甘丹.鲍曼不动杆菌噬菌体AB3的全基因组测序及生物信息学分析[J].陆军军医大学学报(原第三军医大学学报),2013,35(15):1562-1565. DOI：

　Zhang Jie,Liu Xi,Gan Dan.Sequencing and bioinformatic analysis of genome of Acinetobacter baumannii bacteriophage AB3[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(15):1562-1565. DOI：

摘要

目的对本研究小组分离的鲍曼不动杆菌噬菌体AB3进行测序和基因组生物信息学分析，阐明其亲缘关系。方法采用鸟枪法和重叠群组装的策略对噬菌体AB3进行基因组测序，并通过EditSeq、tRNAscan-SE、TRF、FindTerm、ORF finder、BPROM、GeneMark

、Clustalx、phylip等软件对所获噬菌体AB3基因组的一般特性、编码基因的功能预测、RNA聚合酶基因系统的进化进行分析。结果噬菌体AB3基因组为全长31 185 bp的双链DNA，G+C含量为39.18%，包含28个预测基因，1个转录终止子和4个可能的启动子序列。结论基因分析和RNA聚合酶基因进化分析显示噬菌体AB3与噬菌体AB1类似，均属于phiKMV-like病毒属。

Abstract

Objective To sequence the Acinetobacter baumannii bacteriophage AB3 separated by our team

and to perform bioinformatics analysis

so as to identify the classification of its phylogenetic relationship. Methods Shot-gun library and config package strategy were carried out for sequencing the genome of bacteriophage AB3. Such software as EditSeq

tRNAscan-SE

TRF

FindTerm

ORF finder

BPROM and GeneMark

were applied to predict both general characteristics of the bacteriophage AB3 genome and the coding gene function. In addition

the evolution of RNA polymerase gene system was analyzed with the software of Clustalx and phylip. Results The genome of bacteriophage AB3 was a

double-strand DNA with a full length of 31 185 bp

in which G+C mol% was 39.18% and 28 predicted genes

1 transcription terminator

and 4 possible promoter sequences were included. Conclusion Genetic analysis and RNA polymerase gene evolution analysis indicate that bacteriophage AB3 is similar to bacteriophage AB1

and both of them belong to phiKMV-like virus.

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Related Institution

School of Medicine， Chongqing University

Department of Gastroenterology， Chongqing University Cancer Hospital

Department of Gastroenterology， Chongqing Key Laboratory of Precise Prevention and Treatment of Digestive Malignancies， Army Medical Center of PLA/Daping Hospital of Third Military Medical University

Department of Oncology， Chongqing Hospital of Traditional Chinese Medicine

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