Objective      To determine the tissue expression profile of human TRAF3IP3 gene

also known as TRAF3-interacting Jun N-terminal kinase-activating modulator (T3JAM) and its subcellular localization.       Methods      The mRNA expression of TRAF3IP3 was detected in 11 human tissue samples

including the liver

thyroid

colon

spleen

stomach

testis

uterus

fat tissue

bone marrow

lung and lymph node by real-time PCR. The subcellular location plasmid containing the full-length TRAF3IP3 cDNA was constructed. And then the recombinant plasmid was transfected into HEK 293 cells. Subcellular location of TRAF3IP3 was detected by confocal laser scanning microscopy.       Results      TRAF3IP3 mRNA was expressed in all detected human tissues

and highly expressed in the lymph node

spleen

bone marrow

stomach and testis. The human TRAF3IP3 eukaryotic expression vector was constructed successfully. Confocal laser scanning microscopy showed TRAF3IP3 protein was located in the nuclear membrane

in a perinuclear granular distribution.       Conclusion      TRAF3IP3 mRNA is highly expressed in the lymphocyte-richened tissues

and TRAF3IP3 protein is mainly located in the nuclear membrane and perinuclear region

which might be involved in the development

maturation and regulation of immune system.