Silencing integrin-linked kinase inhibits growth and metastasis in human tongue cancer TCA8113 cells

幸宇; 邓世雄; 陈俊霞

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Silencing integrin-linked kinase inhibits growth and metastasis in human tongue cancer TCA8113 cells

更新时间：2026-01-09

- Silencing integrin-linked kinase inhibits growth and metastasis in human tongue cancer TCA8113 cells
- Journal of Army Medical University Vol. 35, Issue 15, Pages: 1552-1557(2013)
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  CLC： R394.3;R73-37;R739.86
- Published：2013
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幸宇,邓世雄,陈俊霞.沉默整合素连接激酶基因抑制TCA8113舌癌细胞生长和侵袭[J].陆军军医大学学报(原第三军医大学学报),2013,35(15):1552-1557.

　Xing Yu,Deng Shixiong,Chen Junxia.Silencing integrin-linked kinase inhibits growth and metastasis in human tongue cancer TCA8113 cells[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(15):1552-1557.
幸宇,邓世雄,陈俊霞.沉默整合素连接激酶基因抑制TCA8113舌癌细胞生长和侵袭[J].陆军军医大学学报(原第三军医大学学报),2013,35(15):1552-1557. DOI：

　Xing Yu,Deng Shixiong,Chen Junxia.Silencing integrin-linked kinase inhibits growth and metastasis in human tongue cancer TCA8113 cells[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(15):1552-1557. DOI：

摘要

目的研究沉默整合素连接激酶（integrin-linked kinase，ILK）基因表达，对人舌鳞癌细胞生长、侵袭和转移能力的影响。方法通过用ILK mRNA的特异性siRNA表达载体和无同源性的对照载体，在脂质体介导下稳定转染人舌鳞癌TCA8113细胞，分为TCA8113组、TCA8113 vector组和TCA8113 siILK组，通过筛选鉴定后，用MTT法、划痕试验和Transwell法分别检测细胞生长、迁移和侵袭能力，用Western blot分析细胞中ILK、p-Akt、p-GSK3β及Snail等的表达。结果沉默ILK基因表达显著抑制了细胞的生长、迁移和侵袭能力，接种的第48、72、96、120小时，TCA8113 siILK组的抑制率分别为17%、29%、25%、42%，迁移能力较TCA8113组和TCA8113 vector组分别下降67%和66%，侵袭能力则较两个对照组下降了67%和68%， p-Akt、p-GSK3β及Snail的表达较TCA8113组和TCA8113 vector组分别降低了45%和53%，57%和61%，74%和73%。结论抑制ILK基因的表达可通过Akt/GSK3β/Snail途径，显著抑制人舌鳞癌TCA8113细胞的生长、迁移和侵袭潜能，可作为治疗人舌鳞癌的靶蛋白。

Abstract

Objective To investigate the effect of silencing integrin-linked kinase (ILK) on the proliferation

migration and invasion of human tongue squamous cancer TCA8113 cells. Methods ILK siRNA expression plasmid and a non-homologous vector (negative control) were transferred into TCA8113 cells

respectively. Then the transfected cells were screened and identified. MTT assay

wound healing experiment and transwell assay were employed to measure cell proliferation

migration and invasion. The expression of p-Akt

p-GSK3β and Snail was detected by Western blotting. Results Inhibition of ILK expression obviously suppressed TCA8113 cell proliferation

migration and invasion

as well as down-regulated the expression of p-Akt

p-GSK3β and Snail. The inhibitory rates at 48

96 and 120 h were 17%

29%

25% and 42% in TCA8113 siILK group

respectively. The migration capacity was decreased by 67% and 66% compared with the other two control groups. The invasion capacity was decreased by 67% and 68% compared with the other two control groups. The expression of p-Akt

p-GSK3β and Snail was decreased by 45% and 53%

57% and 61%

74% and 73%

compared with the other two control groups

respectively. Conclusion Silencing ILK inhibits the proliferation

migration and invasion of TCA8113 cells through Akt/GSK3β/Snail-dependent pathway. ILK can be a therapeutic target protein for tongue cancer.

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Related Author

幸宇

齐进

邓世雄

陈俊霞

刘浩

曹友德

叶维霞

孙阳阳

Related Institution

Department of Gastroenterology, Qingdao Central Hospital of Rehabilitation University

Endoscopy Center, Qingdao Central Hospital of Rehabilitation University

Department of Pathology， Second Affiliated Hospital， Army Medical University （Third Military Medical University）

School of Medicine， Shihezi University

Department of Stomatology， the First Affiliated Hospital of Shihezi University

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