miRNA-144 inhibiting osteogenesis of mesenchymal stem cells by targeting cadherin-11

康夏; 康菲; 杨波; 郭洪峰; 权毅; 董世武

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miRNA-144 inhibiting osteogenesis of mesenchymal stem cells by targeting cadherin-11

更新时间：2026-01-09

- miRNA-144 inhibiting osteogenesis of mesenchymal stem cells by targeting cadherin-11
- Journal of Army Medical University Vol. 35, Issue 10, Pages: 922-926(2013)
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  CLC： R322.71;R329.2;R394.3
- Published：2013
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康夏,康菲,杨波,郭洪峰,权毅,董世武.miRNA-144靶向调节钙粘蛋白11对间充质干细胞成骨分化的抑制效应[J].陆军军医大学学报(原第三军医大学学报),2013,35(10):922-926.

　Kang Xia,Kang Fei,Yang Bo,Guo Hongfeng,Quan Yi,Dong Shiwu.miRNA-144 inhibiting osteogenesis of mesenchymal stem cells by targeting cadherin-11[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(10):922-926.
康夏,康菲,杨波,郭洪峰,权毅,董世武.miRNA-144靶向调节钙粘蛋白11对间充质干细胞成骨分化的抑制效应[J].陆军军医大学学报(原第三军医大学学报),2013,35(10):922-926. DOI：

　Kang Xia,Kang Fei,Yang Bo,Guo Hongfeng,Quan Yi,Dong Shiwu.miRNA-144 inhibiting osteogenesis of mesenchymal stem cells by targeting cadherin-11[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(10):922-926. DOI：

摘要

目的证实miRNA-144靶向调节钙粘蛋白11(Cad-11)表达，抑制间充质干细胞（mesenchymal stem cell，MSCs）的成骨分化。方法通过双荧光素酶报告系统对Cad-11与miRNA-144的靶基因关系进行鉴定；将pre-miR-144及anti-miR-144分别转染MSCs，成骨诱导7、14 d后，实时定量PCR和蛋白印迹方法检测细胞中Cad-11及成骨标志物Runx2、ALP的表达情况，分析其与MSCs成骨分化的变化关系。黏附实验检测miRNA-144对MSCs成骨分化过程中黏附的影响。结果 Cad-11是miRNA-144的靶基因；miRNA-144在成骨诱导第7、14天可明显抑制Cad-11、Runx2和ALP的表达(P0.05)，同时降低MSCs的黏附性。结论 miRNA-144通过抑制Cad-11的表达对间充质干细胞的成骨分化起负向调控的作用。

Abstract

Objective To investigate the role of miRNA-144 in inhibiting the osteogenesis of mesenchymal stem cells (MSCs) by regulating cadherin-11 (Cad-11). Methods Dual-luciferase reporter assay was used to identify the relationship between miRNA-144 and Cad-11. Pre-mir-144 and anti-mir-144 were transferred into C3H/10T1/2 cells

respectively. Then the cells were transfected after osteogenesis induction for 7 d and 14 d. Real-time PCR and Western blotting were performed to detect the expression of Cad-11 and related osteogenetic biomarkers

including Runx2 and ALP. Then the adhesion of MSCs was tested. Results Cad-11 was the target gene of miRNA-144

and miRNA-144 could downregulate the expression of Cad-11

Runx2 and ALP. Furthermore

it could inhibit the adhesion of MSCs. Conclusion miRNA-144 can inhibit the osteogenesis process of MSCs by downregulating Cad-11.

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