Inhibition of Klotho on epithelia-mesenchymal transition of renal tubule epithelial cells induced by FGF2

管旭; 杨可; 肖堂利; 李艺; 何婷; 徐新丽; 赵景宏

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Inhibition of Klotho on epithelia-mesenchymal transition of renal tubule epithelial cells induced by FGF2

更新时间：2026-01-09

- Inhibition of Klotho on epithelia-mesenchymal transition of renal tubule epithelial cells induced by FGF2
- Journal of Army Medical University Vol. 35, Issue 13, Pages: 1327-1330(2013)
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  CLC： R322.61;R329.24;R394.2
- Published：2013
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管旭,杨可,肖堂利,李艺,何婷,徐新丽,赵景宏.Klotho对FGF2诱导的肾小管上皮细胞上皮-间充质转分化的抑制作用[J].陆军军医大学学报(原第三军医大学学报),2013,35(13):1327-1330.

　Guan Xu,Yang Ke,Xiao Tangli,Li Yi,He Ting,Xu Xinli,Zhao Jinghong.Inhibition of Klotho on epithelia-mesenchymal transition of renal tubule epithelial cells induced by FGF2[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(13):1327-1330.
管旭,杨可,肖堂利,李艺,何婷,徐新丽,赵景宏.Klotho对FGF2诱导的肾小管上皮细胞上皮-间充质转分化的抑制作用[J].陆军军医大学学报(原第三军医大学学报),2013,35(13):1327-1330. DOI：

　Guan Xu,Yang Ke,Xiao Tangli,Li Yi,He Ting,Xu Xinli,Zhao Jinghong.Inhibition of Klotho on epithelia-mesenchymal transition of renal tubule epithelial cells induced by FGF2[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(13):1327-1330. DOI：

摘要

目的探讨Klotho抑制成纤维细胞生长因子2（fibroblast growth factor2，FGF2）诱导的肾小管上皮细胞（HK-2）上皮-间充质转分化（epithelia-mesenchymal transition，EMT）的作用及机制。方法重组人Klotho蛋白预孵育前后，倒置显微镜观察FGF2对HK-2细胞形态的影响，免疫荧光检测上皮细胞标志物cytokeratin和间充质细胞标志物vimentin 的表达变化，Western blot检测α-SMA、E-cadherin和ERK1/2的表达变化。结果 100 ng/mL的FGF2作用于HK-2 细胞48 h后，HK-2细胞变成长梭形，细胞间隙明显增大，上皮细胞标志物E-cadherin的表达明显下降，间充质细胞标志物α-SMA表达明显升高（

0.05），提示FGF2可以诱导HK-2细胞发生EMT。而Klotho蛋白预孵育可以显著抑制FGF2诱导的EMT，并且FGF2/FGFR（FGFs受体）下游信号分子pERK1/2的表达明显减弱（

0.05）。结论 Klotho可以通过减弱FGF2信号通路活化抑制FGF2诱导的HK-2细胞EMT，Klotho可能参与了肾间质纤维化的发生和发展。

Abstract

Objective To determine the effect and mechanism of Klotho on the epithelia-mesenchymal transition (EMT) of HK-2 cells induced by fibroblast growth factor 2 (FGF2). Methods The effect of FGF2 on HK-2 cells morphology with and without pre-incubation of Klotho was observed. The expression levels of epithelial marker cytokeratin and mesenchymal marker vimentin were detected by immunofluorescence

and the expression levels of α-smooth muscle actin (α-SMA)

E-cadherin and ERK1

/2 were detected by Western blotting. Results After incubated with FGF2 (100 ng/mL) for 48 h

HK-2 cells turned into long-spindled shape

and the intercellular space markedly increased. The expression of epithelial marker E-cadherin significantly decreased (

0.05)

while the mesenchymal marker α-SMA increased (

0.05)

indicating that FGF2 could induce EMT in HK-2 cells. The pre-incubation of Klotho could markedly inhibit the EMT induced by FGF2 and the expression of pERK1/2 in HK-2 cells (

0.05). Conclusion Klotho can inhibit FGF2-induced EMT via attenuating the activation of FGF2 signaling pathway

and may participate in the development of renal interstitial fibrosis.

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