Objective　　　To investigate the phenotype and metergasis of dendritic cells (DCs) in peripheral blood of patients with bladder carcinoma.　　　 Methods　　　DCs were isolated from the peripheral blood of patients with bladder carcinoma and healthy subjects by density gradient centrifugation

respectively

and then the 2 groups of DCs were cultured in vitro with rhGM-CSF and rhIL-4 induction. The expression levels of PD-L1

CD1a

HLA and CD83 on the DCs were detected by flow cytometry

and the proliferation of lymphoid cells stimulated by the 2 groups of DCs were

tested by mixed lymphocyte reaction. ELISA was used to measured the levels of IL-10 and IL-12 secreted by the 2 groups of DCs.　　　 Results　　　 The expression level of PD-L1 [(95.06±4.06)% vs (76.63±6.90)%

]

and the content of IL-10 secreted by DCs (214.00±13.75 

vs

83.78±7.95 pg/mL) were significantly higher in the bladder carcinoma patients than in the normal control

whereas

CD83 expression level was significantly reduced [(16.20±1.91)% 

vs

(35.53±1.58)%

P0.05

]

. Moreover

the proliferation of lymphoid cells stimulated with DCs was decreased in patient group than in healthy subject group.　　　 Conclusion　　　Bladder carcinoma patients’ DCs have a phenotype of over-expressing PD-L1

over-secreting IL-10 and down-expressing CD83. The changed phenotype may be one of possible mechanisms of immune evasion of the tumor.