Objective      To evaluate the therapeutic effectiveness of intrastromal amphotericin B (AmB) injection in the treatment of refractory fungal keratohelcosis in order to explore a new way for topical treatment.       Methods      A total of 6 eyes from 6 patients who were diagnosed as fungal keratohelcosis and had no obvious response to a over-2-week treatment of topical antifungal eye-drops were enrolled in the study. They were given intrastromal injection of AmB (7.5 μg in 0.1 ml) at the periphery clear cornea with a 30-gauge patented corneal stroma injection needle

once every 3 d

and were also treated with topical antifungal eye-drops. The clinical efficacy and outcomes were assessed after the treatment and during follow-up.       Results      Six patients included 3 males and 3 females

with a mean age of 46.0±13.6

and their mean course of disease was 9.8 weeks. Before the intrastromal injection

their best-corrected visual acuity (BCVA) ranged from hand movement (HM) to 0.1. After giving 3 to 5 times of intrastromal injections for each patient

VA of 4 patients was increased greatly

and that of the other 2 maintained the same. The BCVA reached from CF/10cm to 0.4. All eyes healed with corneal scar formation

and no serious complication occurred during the 6 months’ follow-ups.       Conclusion      Intrastromal injection of AmB is an effective and safe way for refractory fungal keratohelcosis.