miR-18a enhancing radiosensitivity of A549 cells and its molecular mechanism

吴磊; 孙建国; 徐睿; 李德志; 徐燕梅; 马虎; 李雪涛; 彭丽娜; 陈正堂

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miR-18a enhancing radiosensitivity of A549 cells and its molecular mechanism

更新时间：2026-01-09

- miR-18a enhancing radiosensitivity of A549 cells and its molecular mechanism
- Journal of Army Medical University Vol. 35, Issue 9, Pages: 870-873(2013)
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  CLC： R394.6;R730.55;R734.2
- Published：2013
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吴磊,孙建国,徐睿,李德志,徐燕梅,马虎,李雪涛,彭丽娜,陈正堂.miR-18a对A549细胞的放射增敏作用及其机制[J].陆军军医大学学报(原第三军医大学学报),2013,35(09):870-873.

　Wu Lei,Sun Jianguo,Xu Rui,Li Dezhi,Xu Yanmei,Ma Hu,Li Xuetao,Peng Lina,Chen Zhengtang.miR-18a enhancing radiosensitivity of A549 cells and its molecular mechanism[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(09):870-873.
吴磊,孙建国,徐睿,李德志,徐燕梅,马虎,李雪涛,彭丽娜,陈正堂.miR-18a对A549细胞的放射增敏作用及其机制[J].陆军军医大学学报(原第三军医大学学报),2013,35(09):870-873. DOI：

　Wu Lei,Sun Jianguo,Xu Rui,Li Dezhi,Xu Yanmei,Ma Hu,Li Xuetao,Peng Lina,Chen Zhengtang.miR-18a enhancing radiosensitivity of A549 cells and its molecular mechanism[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(09):870-873. DOI：

摘要

目的　　　观察miR-18a对A549肺腺癌细胞放射敏感性的影响。　　　方法　　　 qRT-PCR检测2 Gy X线照射A549细胞后miR-18a的变化；将人工合成的miR-18a模拟物（mimics）转染A549细胞，测定不同剂量照射后A549细胞的克隆形成能力，描记细胞存活曲线，测算2 Gy时存活分数（SF

）等指标，判断miR-18a对A549细胞放射敏感性的影响；同时检测过表达miR-18a的A549细胞中共济失调-毛细血管扩张症突变基因（ataxia-telangiectasia mutated，ATM）蛋白，探讨miR-18a对A549细胞放射敏感性影响的分子机制。　　　结果　　　与照射前（1.00±0.04）相比，2 Gy剂量照射后A549细胞miR-18a水平[（0.18±0.04）~（0.31±0.09）

]

显著降低（P0.05）。miR-18a过表达后A549细胞放射敏感性增强，miR-18a过表达组、空白对照组SF

分别为0.34，0.48；同时miR-18a过表达的A549细胞中ATM、磷酸化ATM（ATM phospho S1981）水平下降。　　　结论　　　 miR-18a上调A549细胞放疗敏感性，可能与miR-18a下调ATM有关。

Abstract

Objective　　　 To determine the effect of miR-18a on the radiosensitivity of A549 cells and investigate the underlying molecular mechanisms.　　　 Methods　　　 After A549 cells were treated with 2 Gy X-ray

miR-18a was determined by qRT-PCR. And then miR-18a mimics were transfected into A549 cells

after exposure to X-ray radiation at different doses

surrival fraction was assessed by clonogenic assay. Survival fraction at 2 Gy (SF

) and sensitive enhancement ration (SER) were calculated. The protein levels of

ataxia telangiectasia mutated (ATM) kinase and ATM (phospho S1981) were determined by Western blotting after up-regulating miR-18a.　　　 Results　　　 miR-18a was decreased from 1.00±0.04 to 0.18±0.04-0.31±0.09 in A549 cells after exposure to 2 Gy X-ray (P0.05). miR-18a over-expression increased the radiosensitivity in A549 cells. The SF

in miR-18a over-expression cells and normal A549 cells was 0.34 and 0.48

respectively. Moreover

the expression levels of ATM and ATM (phospho S1981) were down-regulated in miR-18a over-expressed A549 cells.　　　 Conclusion　　　 In A549 cells

miR-18a enhances radiosensitivity by inhibiting ATM and ATM (phospho S1981).

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