Lymphocyte particles stimulates AKT/Foxo1 blocking airway epithelial cell cycle in vitro

焦燕; 邱倩; 杨再兴; 但小苹; 熊玮

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Lymphocyte particles stimulates AKT/Foxo1 blocking airway epithelial cell cycle in vitro

更新时间：2026-01-09

- Lymphocyte particles stimulates AKT/Foxo1 blocking airway epithelial cell cycle in vitro
- Journal of Army Medical University Vol. 34, Issue 24, Pages: 2498-2502(2012)
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  CLC： R392.11;R392.32;R560.2
- Published：2012
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焦燕,邱倩,杨再兴,但小苹,熊玮.淋巴细胞微粒刺激AKT/Foxo1阻滞气道上皮细胞周期的研究[J].陆军军医大学学报(原第三军医大学学报),2012,34(24):2498-2502.

　JiaoYan,Qiu Qian,Yang Zaixing,Dan Xiaoping,Xiong Wei.Lymphocyte particles stimulates AKT/Foxo1 blocking airway epithelial cell cycle in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(24):2498-2502.
焦燕,邱倩,杨再兴,但小苹,熊玮.淋巴细胞微粒刺激AKT/Foxo1阻滞气道上皮细胞周期的研究[J].陆军军医大学学报(原第三军医大学学报),2012,34(24):2498-2502. DOI：

　JiaoYan,Qiu Qian,Yang Zaixing,Dan Xiaoping,Xiong Wei.Lymphocyte particles stimulates AKT/Foxo1 blocking airway epithelial cell cycle in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(24):2498-2502. DOI：

摘要

目的研究淋巴细胞微粒(lymphocyte microparticles，LMPs)作用于气道上皮细胞引起周期阻滞在G

期的机制通路。方法将人类正常气道上皮细胞培养到对数生长期时，20 μg/ml的LMPs按照不同的时间0、4、8、16、20、24 h刺激气道上皮细胞，用RT-PCR方法检测P21、P27在mRNA水平的表达情况。20 μg/ml的LMPs按照不同的时间0、4、8、16、24 h刺激气道上皮细胞，用Western blot 方法检测P21、P27、Foxo1、p-Foxo1、AKT、p-AKT的蛋白表达情况。用免疫细胞化学技术检测Foxo1的核定位情况。结果与0 h对比，20 μg/ml的LMPs作用于气道上皮细胞能使P21、P27的mRNA、蛋白水平显著升高（P0.01），Foxo1蛋白水平无明显变化(P0.05)，p-Foxo1的蛋白水平表达减弱(P0.01)，AKT蛋白水平无明显变化(P0.05)，p-AKT蛋白水平表达减弱(P0.01)。结论 LMPs作用于气道上皮细胞上调P21、P27蛋白的表达引起周期阻滞G

期

可能是通过AKT/Foxo1信号通路调节。

Abstract

Objective To determine the effect of lymphocyte microparticles (LMPs) on the cell cycle of airway epithelial cells and investigate the underlying mechanism. Methods LMPs of 20 μg/ml was treated human normal airway epithelial cells at logarithmic growth period for 0

20 and 24 h respectively. RT-polymerase chain reaction (PCR) was used to detect the expression of P21 and P27 at mRNA level. Western blot analysis was employed to test the expression of P21

P27

Foxo1

p-Foxo1

AKT and p-AKT at protein level. I

mmunocytochemical assay was to test the nuclear localization of Foxo1. Results LMPs of 20 μg/ml resulted in an obvious increase of P21 and P27 at mRNA and protein levels (P0.01). No change was found in Foxo1 and AKT protein levels (P0.05)

but their phosphorylated proteins were evidently down-regulated (P0.01). Conclusion LMPs upregulates P21 and P27 expression and causes cell cycle arrested at G

phase in the airway epithelial cells through AKT/Foxo1 signaling pathways.

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