Expression of DNA damage repair genes, thymidylate synthase and β-tubulin Ⅲ in human osteosarcoma tissues and its relationship with clinical pathology of osteosarcoma

蒋平; 关伟; 戴楠; 仲召阳; 毛承毅; 顾咸庆; 王东

您当前的位置：

首页 >

文章列表页 >

Expression of DNA damage repair genes, thymidylate synthase and β-tubulin Ⅲ in human osteosarcoma tissues and its relationship with clinical pathology of osteosarcoma

更新时间：2026-01-09

- Expression of DNA damage repair genes, thymidylate synthase and β-tubulin Ⅲ in human osteosarcoma tissues and its relationship with clinical pathology of osteosarcoma
- Journal of Army Medical University Vol. 35, Issue 1, Pages: 61-65(2013)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R394.2;R730.23;R738.1
- Published：2013
- 稿件说明：
移动端阅览
蒋平,关伟,戴楠,仲召阳,毛承毅,顾咸庆,王东.DNA损伤修复基因以及TS、β-tubulinⅢ在骨肉瘤中的表达及其与临床病理的关系[J].陆军军医大学学报(原第三军医大学学报),2013,35(01):61-65.

　Jiang Ping,Guan Wei,Dai Nan,Zhong Zhaoyang,Mao Chengyi,Gu Xianqing,Wang Dong.Expression of DNA damage repair genes, thymidylate synthase and β-tubulin Ⅲ in human osteosarcoma tissues and its relationship with clinical pathology of osteosarcoma[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(01):61-65.
蒋平,关伟,戴楠,仲召阳,毛承毅,顾咸庆,王东.DNA损伤修复基因以及TS、β-tubulinⅢ在骨肉瘤中的表达及其与临床病理的关系[J].陆军军医大学学报(原第三军医大学学报),2013,35(01):61-65. DOI：

　Jiang Ping,Guan Wei,Dai Nan,Zhong Zhaoyang,Mao Chengyi,Gu Xianqing,Wang Dong.Expression of DNA damage repair genes, thymidylate synthase and β-tubulin Ⅲ in human osteosarcoma tissues and its relationship with clinical pathology of osteosarcoma[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(01):61-65. DOI：

摘要

目的探讨人骨肉瘤组织中DNA损伤修复基因以及TS、β-tubulinⅢ多个化疗反应相关标志物的表达谱特点，及其与临床病理的关系。方法选取50例诊断明确的骨肉瘤患者组织蜡块，运用组织芯片和免疫组化技术检测OGG1、BRCA1、RRM1、XRCC1、APE1、ERCC1以及TS、β-tubulinⅢ 8种基因在骨肉瘤组织中的表达情况。结果 ①OGG1、BRCA1、RRM1、XRCC1、APE1、ERCC1在骨肉瘤组织中呈阳性表达，阳性率分别为96%、94%、94%、84%、84%、74%，强阳性率为64%、76%、54%、72%、48%、2%，而TS仅有6例有弱阳性表达，β-tubulinⅢ无阳性表达。②阳性表达的基因中，仅APE1表达与骨肉瘤的组织病理类型具有相关性（P0.05）。③等级相关分析，APE1和RRM1、APE1和OGG1、OGG1和BRCA1表达呈正相关（r=0.342、0.318、0.319，P0.05），BRCA1和ERCC1表达呈负相关（r=-0.324，P0.05）；APE1和XRCC1、RRM1和XRCC1的表达也呈正相关（r=0.406、0.677，P0.01）。结论骨肉瘤组织中存在多种DNA损伤修复相关基因的表达，其中部分基因的表达强弱之间具有相关性，APE1的表达强弱与肺癌病理类型有关。

Abstract

Objective To explore the expression profiles of multiple chemotherapy-related markers of DNA damage repair genes and thymidylate synthase (TS) β-tubulin Ⅲ in human osteosarcoma tissues and their relationship with clinical pathology of osteosarcoma. Methods Fifty cases of osteosarcoma tissue paraffin blocks were obtained from the patients with diagnosed osteosarcoma. Tissue microarray and immunohistochemical method were applied to detect gene expression levels of OGG1

BRCA1

RRM1

XRCC1

APE1

ERCC1

TS and β-tubulin Ⅲ in the osteosarcoma tissues. Results ①OGG1

BRCA1

RRM1

XRCC1

APE1 and ERCC1 were positively expressed in osteosarcoma tissues with the positive rates of 96%

94%

84%

84% and 74%

respectively

and their strongly positive rates were 64%

76%

54%

72%

48% and 2%

respectively. There were only 6 cases showed weakly positive expression of TS

and β-tubulin Ⅲ was negative in osteosarcoma tissues. ②In the positively expressed genes

only APE1 expression was correlated with osteosarcoma pathological types (P0.05). ③The results of rank correlation analysis showed that APE1 expression was positively correlated with the expression of RRM1

OGG1 and XRCC1 (P0.05

P0.01)

OGG1 expression was positively correlated with BRCA1 expression (P0.05)

RRM1 expression was positively correlated with and XRCC1 expression (P0.01)

but the expression of BRCA1 was negatively correlated with ERCC1 expression (P0.05). Conclusion Several types of DNA damage repair genes are expressed in osteosarcoma tissues

and some of their expression levels have Spearman rank correlations. The characteristics of the chemotherapy-related gene expression in osteosarcoma may have significance for individual chemotherapy in patients with osteosarcoma.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Expression of P504s and claudin-4 in prostate cancer and prognostic value of their combined detection

Expression of miR-616 in osteosarcoma and its role in proliferation, apoptosis, migration and invasion of tumor cells

Application of 3D-printed customized prostheses in limb-salvage surgery for pediatric osteosarcoma of the distal femur: a case series study

Endothelial cells promote osteosarcoma cell metastasis by inhibiting LGI4 expression and activating NOTCH4 signaling pathway

Inhibitor of differentiation-1 promotes angiogenesis of human osteosarcoma by regulating VEGF-A

Related Author

毕罡

毛成毅

江军

张尧

张军

龙吟

FU Wanlei

HAO Xianglin

Related Institution

Department of Pathology， Second Affiliated Hospital， Army Medical University （Third Military Medical University）

Department of Orthopedics， Affiliated Children’s Hospital， Chongqing Medical University

Department of Pediatric Oncology, Institute of Pediatrics, Key Laboratory of Child Development and Disorders of Ministry of Education, Chongqing Key Laboratory of Pediatrics,Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Children's Hospital of Chongqing Medical University

Third Department of Orthopaedics, Second Hospital, Lanzhou University

⁰