Role of Osterix in development of spine in mice

宗兆文; 陈思旭; 贾敏; 沈岳; 赵玉峰; 郭庆山; 华翔; Jerry Feng

您当前的位置：

首页 >

文章列表页 >

Role of Osterix in development of spine in mice

更新时间：2026-01-09

- Role of Osterix in development of spine in mice
- Journal of Army Medical University Vol. 35, Issue 3, Pages: 220-222(2013)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R336;R394.1;R682.3
- Published：2013
- 稿件说明：
移动端阅览
宗兆文,陈思旭,贾敏,沈岳,赵玉峰,郭庆山,华翔,Jerry Feng.Osterix在调控脊柱发育中的作用[J].陆军军医大学学报(原第三军医大学学报),2013,35(03):220-222.

　Zong Zhaowen,Chen Sixu,Jia Min,Shen Yue,Zhao Yufeng,Guo Qingshan,Hua Xiang,Jerry Feng.Role of Osterix in development of spine in mice[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(03):220-222.
宗兆文,陈思旭,贾敏,沈岳,赵玉峰,郭庆山,华翔,Jerry Feng.Osterix在调控脊柱发育中的作用[J].陆军军医大学学报(原第三军医大学学报),2013,35(03):220-222. DOI：

　Zong Zhaowen,Chen Sixu,Jia Min,Shen Yue,Zhao Yufeng,Guo Qingshan,Hua Xiang,Jerry Feng.Role of Osterix in development of spine in mice[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(03):220-222. DOI：

摘要

目的观察核转录因子Osterix在脊柱发育中的作用。方法采用Lox P/Cre系统，以2.3 kb Ⅰ型胶原蛋白为启动子，在小鼠成骨细胞和骨细胞中特异性敲除Osterix，观察Osterix敲除小鼠大体情况，采用X线摄片、HE染色和Nissl染色观察其对小鼠脊柱发育的影响。结果 Osterix敲除小鼠后肢瘫痪，行走时前肢运动正常，拖动后肢爬行，Osterix 的敲除效率约为75%；X线片结果显示，Osterix敲除小鼠形体变小，胸腰段脊柱出现楔形椎体、严重的脊柱侧弯和椎管狭窄等畸形；HE染色结果显示胸腰段脊椎结构发育严重异常；Nissl染色结果显示，受累节段脊髓神经元结构严重受损。结论 Osterix在脊柱发育中起到重要作用，敲除后可导致脊柱侧弯等畸形。

Abstract

Objective To investigate the role of Osterix in spine development. Methods Osterix was knocked out by crossing 2.3 kb Collagen I Cre mice with Osterix Lox P +/+ mice. X-ray radiology

HE staining and Nissl staining were employed to observe the effect of Osterix knockout on spine development. Results Osterix knockout mice demonstrated short stature

wedged vertebrae

severe scoliosis and spinal stenosis at the thoracic and lumbar regions of spine. HE staining showed that the structure of growth plate

vertebral body and spinal canal were totally disorganized. Nissl staining revealed that the neurons in the spinal cord at the inflicted region were strikingly damaged. Conclusion Osterix plays an important role in spine development

and Osterix knockout leads to severe spinal deformities such as scoliosis.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Developmental process and expression patterns of fibroblast growth factor receptors in murine meniscus after birth

Myocardial-specific Hdac3 deletion induces by ventricular remodeling in mice

Influencing factors for reposition of bone fragment in thoracolumbar burst fracture: analysis of 62 patients

Physiological and neurobehavioral changes in newborn mice exposed to cadmium at early life

Construction of targeting vector and preparation of fibrous sheath CABYR-binding protein knockout mice using CRISPR/Cas9 system

Related Author

李芳芳

谢杨丽

黄俊兰

张若彬

蒋宛凌

张大力

许萌

杜晓兰

Related Institution

No data

⁰