Objective      To determine the effect of lily polysaccharides combined with bone marrow mesenchymal stem cells (BMSCs) transplantation on bleomycin-induced pulmonary fibrosis mice.       Methods        BMSCs of mice were cultured in vitro. Ninety healthy SPF mice were randomly divided into a control group (C)

a model group (M)

a bleomycin + lily polysaccharides combined with BMSCs transplantation group (A)

a bleomycin + BMSCs transplantation group (B)

and a bleomycin + lily polysaccharides group (L). The mice of each group were treated with the corresponding measures at 24 h after the mouse models of pulmonary fibrosis were constructed by bleomycin. On the 7th

14th and 28th day

the mice were sacrificed and the pathological scores of lung tissues were determined by HE staining. On the 28th day

the lung coefficient (LC) and hydroxyproline (HYP) content were determined

lung collagen deposition was observed by Masson trichrome staining

and the expression levels of TNF-α and NF-κB in lung tissues was measured by Western blot analysis.       Results        There were significant differences in the LC

HYP content

pathological score

and the expression levels of TNF-α and NF-κB in lung tissues between group C and group M (P0.05). Compared with group M

those indices significantly decreased in group A and group L (P0.05)

and the pathological score on the 28th day significantly decreased in group B (P0.05). The collagen deposition in group M was greater than that in group A

group L and group B.       Conclusion        Lily polysaccharides combined with BMSCs transplantation can reduce the expression levels of TNF-α and NF-κB in lung tissues and pulmonary pathological changes in bleomycin-induced pulmonary fibrosis mice. The effect of combined intervention is better than that of lily polysaccharides or BMSCs transplantation alone.