Exosomes derived from renal cancer cells induce Jurkat T cell apoptosis in vitro

杨林; 吴小候; 罗春丽; 王丹; 陈力学

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Exosomes derived from renal cancer cells induce Jurkat T cell apoptosis in vitro

更新时间：2026-01-09

- Exosomes derived from renal cancer cells induce Jurkat T cell apoptosis in vitro
- Journal of Army Medical University Vol. 35, Issue 5, Pages: 426-430(2013)
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  CLC： R730.23;R730.3;R737.11
- Published：2013
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杨林,吴小候,罗春丽,王丹,陈力学.肾癌细胞来源的exosomes诱导Jurkat T细胞凋亡[J].陆军军医大学学报(原第三军医大学学报),2013,35(05):426-430.

　Yang Lin,Wu Xiaohou,Luo Chunli,Wang Dan,Chen Lixue.Exosomes derived from renal cancer cells induce Jurkat T cell apoptosis in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(05):426-430.
杨林,吴小候,罗春丽,王丹,陈力学.肾癌细胞来源的exosomes诱导Jurkat T细胞凋亡[J].陆军军医大学学报(原第三军医大学学报),2013,35(05):426-430. DOI：

　Yang Lin,Wu Xiaohou,Luo Chunli,Wang Dan,Chen Lixue.Exosomes derived from renal cancer cells induce Jurkat T cell apoptosis in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(05):426-430. DOI：

摘要

目的体外研究肾癌786-0细胞来源的exosomes介导肿瘤免疫逃逸的机制。方法采用CCK-8法检测肾癌786-0细胞来源的exosomes对Jurkat T细胞生长的影响，瑞氏-姬姆萨染色检测Jurkat T细胞形态变化，Annexin V-FITC/PI双染色流式细胞术检测Jurkat T细胞凋亡率，ELISA法检测Jurkat T细胞分泌功能，可溶性Fas阻断实验检测exosomes对Jurkat T细胞凋亡率的影响，Western blot检测exosomes中FasL、及Jurkat T细胞caspase、Bax及Bcl-2蛋白的表达。结果肾癌786-0细胞来源的exosomes可抑制Jurkat T细胞生长，10 μg/mL exosomes作用于Jurkat T细胞24 h，生长抑制率为(19.64±0.92)%，72 h为(36.24±1.12)%；400 μg/mL exosomes作用24 h，生长抑制率为(55.96±1.35)%，72 h为(76.51±1.37)%。Exosomes诱导Jurkat T细胞凋亡，10 μg/mL exosomes作用于Jurkat T细胞8 h，凋亡率为(7.31±1.32)%，24 h为(20.19±1.47)%；400 μg/mL exosomes作用8 h，凋亡率为(27.28±1.29)%，24 h为(41.72±0.88)%。Exosomes还明显抑制Jurkat T细胞IL-2、IFN-γ、IL-6、IL-10的分泌水平；exosomes高表达FasL，可溶性Fas阻断实验能逆转Jurkat T细胞的凋亡；凋亡诱导过程中caspase-3、caspase-8、caspase-9被激活，Bax/Bcl-2上调。结论肾癌786-0细胞分泌的exosomes能诱导Jurkat T细胞凋亡，介导肿瘤免疫逃逸。

Abstract

Objective To investigate the underlying mechanism of exosomes derived from renal cancer cell lines 786-0 to mediate tumor immune escape in vitro. Methods CCK-8 assay was used to determine the effects of exosomes on proliferation in Jurkat T cells. Morphological changes were by wright-giemsa staining;flow cytometry with Annexin V-FITC/PI double staining was used to detect the apoptosis;secretion functions of Jurkat T cell were detected by ELISA assay; effects of exosomes on apoptosis of Jurkat T cell were detected by soluble Fas block experiment; effects on the protein expression of FasL

caspase

Bax and Bcl-2 were assessed by Western blot analysis. Results Exosomes could inhibit Jurkat T cell proliferation

10 μg/mL exosomes act on Jurkat T cell for 24 and 72 h

growth inhibition rate was (19.64±0.92)% and (36.24±1.12)%; while 400 μg/mL exosomes act on it for 24 h and 72 h

growth inhibition rate was (55.96±1.35)% and (76.51±1.37)% respectively. Exosomes could induce Jurkat T cell apoptosis

10 μg/mL exosomes act on Jurkat T cell for 8 h

apoptosis rate was (7.31±1.32)%

extending this monitoring to 24 h

apoptosis rate was (20.19±1.47)%; while 400 μg/mL exosomes act on it for 8 and 24 h

apoptosis rate was (27.28±1.29)% and (41.72±0.88)% respectively. Exosomes also suppressed IL-2

IFN-γ

IL-6 and IL-10 secretion obviously. FasL was highly expressed in exosomes

soluble Fas block could reverse Jurkat T cell apoptosis. In this course

caspase-3

caspase-8

caspase-9 were activated

and the ratio of Bax/Bcl-2 increased. Conclusion Exosomes could inhibit the growth of Jurkat T cell and induce apoptosis.It could mediate tumor immune escape.

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