Effect of a novel nanosystem of Ac-αCD encapsulating Bcl-xl antisense oligonucleotide on proliferation and apoptosis in pulmonary arterial smooth muscle cells

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Effect of a novel nanosystem of Ac-αCD encapsulating Bcl-xl antisense oligonucleotide on proliferation and apoptosis in pulmonary arterial smooth muscle cells

更新时间：2026-01-09

- Effect of a novel nanosystem of Ac-αCD encapsulating Bcl-xl antisense oligonucleotide on proliferation and apoptosis in pulmonary arterial smooth muscle cells
- Journal of Army Medical University Vol. 35, Issue 9, Pages: 846-849(2013)
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  CLC： R322.121;R329.28;R394.2
- Published：2013
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刘雪萍,贺斌峰,陈华萍,孙欢,杨俊俊,魏征华,窦寅,王关嵩.新型纳米载体Ac-αCD携带的Bcl-xl反义寡核苷酸对肺动脉平滑肌细胞增殖和凋亡的作用[J].陆军军医大学学报(原第三军医大学学报),2013,35(09):846-849.

　Liu Xueping,He Binfeng,Chen Huaping,Sun Huan,Yang Junjun,Wei Zhenghua,Dou Yin,Wang Guansong.Effect of a novel nanosystem of Ac-αCD encapsulating Bcl-xl antisense oligonucleotide on proliferation and apoptosis in pulmonary arterial smooth muscle cells[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(09):846-849.
刘雪萍,贺斌峰,陈华萍,孙欢,杨俊俊,魏征华,窦寅,王关嵩.新型纳米载体Ac-αCD携带的Bcl-xl反义寡核苷酸对肺动脉平滑肌细胞增殖和凋亡的作用[J].陆军军医大学学报(原第三军医大学学报),2013,35(09):846-849. DOI：

　Liu Xueping,He Binfeng,Chen Huaping,Sun Huan,Yang Junjun,Wei Zhenghua,Dou Yin,Wang Guansong.Effect of a novel nanosystem of Ac-αCD encapsulating Bcl-xl antisense oligonucleotide on proliferation and apoptosis in pulmonary arterial smooth muscle cells[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(09):846-849. DOI：

摘要

目的　　　研究纳米载体Ac-αCD携带的Bcl-xl反义寡核苷酸(antisense oligonucleotide

ASON)对大鼠肺动脉平滑肌细胞（rat pulmonary arterial smooth muscle cells，RPASMCs）增殖和凋亡作用。　　　方法　　　设计合成5′端标记Cy3的Bcl-xl ASON，由纳米载体Ac-αCD携带。实验分3组：纳米载体携带的Bcl-xl ASON组（ASON-NPs组）、单纯纳米载体组（NPs组）和空白对照组，分别使用纳米载体Ac-αCD携带的Bcl-xl ASON、纳米载体Ac-αCD和培养液处理RPASMCs 48 h，激光共聚焦显微镜观察RPASMCs对纳米载体携带的Bcl-xl ASON的摄取情况；RT-PCR、Western blot检测Bcl-xl的mRNA和蛋白表达；MTT检测处理后细胞的增殖抑制率；流式细胞仪检测细胞凋亡率。　　　结果　　　激光共聚焦显微镜下可见ASON-NPs组细胞质内大量呈颗粒状均匀分布的红色荧光物质，空白对照组和NPs组细胞细胞质内未见红色荧光物质；ASON-NPs组处理的RPASMCs的Bcl-xl mRNA和蛋白表达显著低于空白对照组和NPs组(P0.05)； ASODN-NPs组、NPs组、空白对照组细胞抑制率分别为：（53.61±3.02）%、（6.30±1.90）%、（1.40±0.62）%，凋亡率分别为：（53.04±2.09）%、（10.98±2.03）%、（2.19±0.11）%、ASON-NPs组和NPs组细胞抑制率、凋亡率均显著高于空白对照组 (P0.01)，ASON-NPs组均显著高于NPs组 (P0.01)。　　　结论　　　纳米载体Ac-αCD携带的Bcl-xl反义寡核苷酸能被RPASMCs有效摄取，从而抑制其增殖，促进凋亡。

Abstract

Objective　　　 To determine the effect of an Ac-αCD nanosystem encapsulating Bcl-xl antisense oligonucleotide (ASON) on the proliferation and apoptosis in pulmonary arterial smooth muscle cells.　　　 Methods　　　 Bcl-xl ASON that had been hallmarked with the Cy3 in 5′-end was synthesized

and then encapsulated into the nanosystem Ac-αCD. Primarily cultured SD rat pulmonary arterial smooth muscle cells were treated by Ac-αCD-Bcl-xl ASON or Ac-αCD for 48 h

and the cells without nanosystem served as control. Confocal microscopy was employed to observe the taking of the nanosystem by the cells. Expression of Bcl-xl at mRNA and protein levels

grow inhibitory rate and apoptotic rate were detected by RT-PCR and Western blotting

MTT assay and flow cytometry

respectively.　　　 Results　　　 There were a great deal of brilliantly red-fluorescent granules distributed evenly in the cytoplasm in the cells treated by Ac-αCD-Bcl-xl ASON. No such red-fluorescent granule was seen in the other 2 kinds of cells. The expression of Bcl-xl at mRNA and protein levels were significantly lower in the cells treated by Ac-αCD-Bcl-xl ASON (P0.05). Cell growth inhibitory rate was (53.61±3.02)% in Ac-αCD-Bcl-xl ASON

(6.30±1.90)% in Ac-αCD and (1.40±0.62)% in control cells. Cell apoptotic rate was (53.04±2.09)% in Ac-αCD-Bcl-xl ASON

(10.98±2.03)% in Ac-αCD and (2.19±0.11)% in control cells

with the former 2 kinds of cells significantly higher than control (P0.01)

so Ac-αCD-Bcl-xl ASON cells than Ac-αCD cells (P0.01).　　　 Conclusion　　　 Our Ac-αCD nanosystem encapsulating Bcl-xl ASON is effectively taken by rat pulmonary arterial smooth muscle cells

and then inhibits cell growth and induces cell apoptosis.

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