Systemic identification of immunodominant Th epitopes from urease B subunit of Helicobacter pylori

李滨; 陈立; 杨武晨; 李海波; 胡健; 何亚非; 章金勇; 赵卓; 吴超

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Systemic identification of immunodominant Th epitopes from urease B subunit of Helicobacter pylori

更新时间：2026-01-09

- Systemic identification of immunodominant Th epitopes from urease B subunit of Helicobacter pylori
- Journal of Army Medical University Vol. 35, Issue 3, Pages: 212-215(2013)
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  CLC： R377;R392-33;R392.11
- Published：2013
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李滨,陈立,杨武晨,李海波,胡健,何亚非,章金勇,赵卓,吴超.幽门螺杆菌尿素酶B亚单位优势Th表位的系统筛选与鉴定[J].陆军军医大学学报(原第三军医大学学报),2013,35(03):212-215.

　Li Bin,Chen Li,Yang Wuchen,Li Haibo,Hu Jian,He Yafei,Zhang Jinyong,Zhao Zhuo,Wu Chao.Systemic identification of immunodominant Th epitopes from urease B subunit of Helicobacter pylori[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(03):212-215.
李滨,陈立,杨武晨,李海波,胡健,何亚非,章金勇,赵卓,吴超.幽门螺杆菌尿素酶B亚单位优势Th表位的系统筛选与鉴定[J].陆军军医大学学报(原第三军医大学学报),2013,35(03):212-215. DOI：

　Li Bin,Chen Li,Yang Wuchen,Li Haibo,Hu Jian,He Yafei,Zhang Jinyong,Zhao Zhuo,Wu Chao.Systemic identification of immunodominant Th epitopes from urease B subunit of Helicobacter pylori[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(03):212-215. DOI：

摘要

目的筛选幽门螺杆菌（Helicobacter pylori

Hp）尿素酶B亚单位（UreB）中CD4

细胞优势应答表位，并确定其MHC限制性。方法以重组UreB蛋白（rUreB）与弗氏佐剂联合皮下多点注射免疫BALB/c小鼠，从小鼠脾脏中体外扩增UreB特异性T淋巴细胞，采用步移重叠合成肽法筛选CD4

T细胞优势应答表位，分析其MHC分子限制性。 结果 18mer短肽筛选发现UreB

403-420

和UreB

409-426

可显著刺激UreB特异性CD4

T细胞产生IFN-γ。13mer短肽鉴定实验结果显示，UreB

409-421

可刺激产生与18mer短肽UreB

403-420

和UreB

409-426

等同的应答强度。同时抗体阻断实验表明，抗H-2

（I-A）单克隆抗体能有效阻断该表位的应答。结论 UreB

403-420

和UreB

409-426

为UreB抗原中的优势Th表位，其刺激免疫反应的核心位置为UreB

409-421

，且存在H-2

（I-A）限制性。

Abstract

Objective To identify systematically MHC-restricted immunodominant T helper lymphocyte (Th) epitopes from urease subunit B of Helicobacter pylori (H. pylori) and to confirm the MHC-restricting profile. Methods BALB/c mice were immunized with recombinant UreB protein (rUreB) and Freund’s adjuvant by subcutaneous injection. Antigen-specific CD4

T cells were amplified in vitro from spleens of immunized mice. The MHC-restricted immunodominant Th epi

topes were identified by synthesizing overlapping peptides and antibody-blocking assay. Results UreB

403-420

and UreB

409-426

induced the strongest secretion of IFN-γ in all the 93 18mer peptides

while 13mer peptide UreB

403-426

within UreB

403-420

and UreB

409-426

induced equivalent responses compared with the 18mer peptides. The results of antibody-blocking assay showed that the H-2

(I-A) monoclonal antibody efficiently blocked the T cell activation induced by peptide UreB

403-426

. Conclusion UreB

403-420

and UreB

409-426

are immunodominant Th epitopes of urease subunit B antigen. The core reactive sequence is UreB

409-421

and its restriction molecule is H-2

(I-A).

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