Effect of TAP-SSL5 fusion protein on binding of human platelets to neutrophils

龚丽莎; 房兆飞; 胡厚源; 刘成海; 孟璟

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Effect of TAP-SSL5 fusion protein on binding of human platelets to neutrophils

更新时间：2026-01-09

- Effect of TAP-SSL5 fusion protein on binding of human platelets to neutrophils
- Journal of Army Medical University Vol. 35, Issue 8, Pages: 754-758(2013)
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  CLC： R331.143;R392.12;R392.3
- Published：2013
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龚丽莎,房兆飞,胡厚源,刘成海,孟璟.融合蛋白TAP-SSL5对血小板与粒细胞结合的影响[J].陆军军医大学学报(原第三军医大学学报),2013,35(08):754-758.

　Gong Lisha,Fang Zhaofei,Hu Houyuan,Liu Chenghai,Meng Jing.Effect of TAP-SSL5 fusion protein on binding of human platelets to neutrophils[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(08):754-758.
龚丽莎,房兆飞,胡厚源,刘成海,孟璟.融合蛋白TAP-SSL5对血小板与粒细胞结合的影响[J].陆军军医大学学报(原第三军医大学学报),2013,35(08):754-758. DOI：

　Gong Lisha,Fang Zhaofei,Hu Houyuan,Liu Chenghai,Meng Jing.Effect of TAP-SSL5 fusion protein on binding of human platelets to neutrophils[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(08):754-758. DOI：

摘要

目的　　　探讨融合蛋白TAP-SSL5对血小板与粒细胞结合作用的影响。　　　方法　　　采用CCK-8试剂盒检测TAP-SSL5对细胞活力的影响；流式细胞仪检测THP-1细胞表面CD162（PSGL-1）的表达，及TAP-SSL5对小鼠抗人CD162单抗KPL-1与THP-1细胞结合的抑制作用。以20 μmol/L ADP激活人血小板，采用流式细胞仪、瑞氏-姬姆萨染色检测血小板与THP-1细胞或人中性粒细胞的结合情况，并研究TAP-SSL5的干预作用。　　　结果　　　TAP-SSL5浓度在30 mg/L或以下时对THP-1细胞或中性粒细胞的活力无明显影响。流式细胞仪检测显示，TAP-SSL5（终浓度10 mg/L）能显著抑制KPL-1与THP-1细胞的结合；20 μmol/L ADP激活的血小板与THP-1细胞或中性粒细胞的结合率分别为（31.3±8.4）%和（35.6±5.9）%；细胞经10 mg/L TAP-SSL5预先处理后，其结合率分别降至（13.4±6.7）%和（10.4±6.4）% （P0.01）。瑞氏-姬姆萨染色的结果表明，TAP-SSL5和SSL5均可抑制激活的血小板与中性粒细胞的结合（P0.05）。　　　结论　　　TAP-SSL5可通过与PSGL-1的结合，而直接抑制激活的血小板与粒细胞的结合。

Abstract

Objective　　　To investigate the effect of anti-inflammatory and anticoagulant tick anticoagulant peptide (TAP)-staphylococcal superantigen like protein-5 (SSL5) fusion protein on the binding of human platelets to neutrophils.　　　 Methods　　　Cell viability was assessed by colorimetric cell counting kit-8 (CCK-8). Flow cytometry (FCM) was applied to detect the expression of CD162 (PSGL-1) on THP-1 cells as well as the inhibitory effect of TAP-SSL5 on the binding of mouse anti-human CD162 monoclonal antibody (KPL-1) to THP-1 cells. Platelets were activated by 20 μmol/L of ADP

and the binding rates of the activated platelets to THP-1 cells and neutrophils were assayed by FCM and Wright-Giemsa staining

respectively.　　　 Results　　　TAP-SSL5≤30 mg/L did not affect the viability of THP-1 cells or human neutrophils

and the TAP-SSL5 at 10 mg/L competitively inhibited KPL-1 binding to THP-1 cells. The binding rates of activated platelets (by 20 μmol/L ADP) to THP-1 cells or neutrophils were (31.3±8.4)% and (35.6±5.9)%

respectively

which decreased to (13.4±6.7)% and (10.4±6.4)% after the THP-1 cells and neutrophils were pre-incubated with 10 mg/L TAP-SSL5 (P0.01). The results of Wright-Giemsa staining showed that both TAP-SSL5 and SSL5 could inhibit the binding of activated platelets to neutrophils (P0.05).　　　 Conclusion　　　TAP-SSL5 can directly inhibit the platelet-neutrophil binding.

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