Objective      To design and prepare a novel bioreactor based on the idea of the quasi-static plane flow field for perfusion culture in order to meet the requirement for culturing engineered-skin structures (ESS).       Methods      A commercial software for computational fluid dynamics (CFD) analysis

FLUENT was used to mimic flow field and wall shear stress (WSS) in the new bioreactor. Based on this

a bioreactor with advantages of stack-up

low stress and homogeneous flat flow field was designed.       Results      The simulation results showed that the maximum WSS on mesh-like support and on the surface of tissue-engineered skin in the growth chamber was less than 3×10

-5

N/cm

2

and the flow field in the

bioreactor was uniform. The new bioreactor remedied some disadvantages in other bioreactors

such as the heterogeneous characteristics of flow

and the excess volume

etc. Our bioreactor met the requirement of gas-liquid interface and low stress for preparation of tissue-engineered skin.       Conclusion      Our new bioreactor satisfies the demands of quasi-static plane flow

and it may be suitable for culturing and producing the tissue-engineered skin in the future.