CA916798 gene is involved in cisplatin resistance in human lung cancer through PI3K/AKT pathway

祝冰晶; 王宇亮; 罗虎; 周向东

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CA916798 gene is involved in cisplatin resistance in human lung cancer through PI3K/AKT pathway

更新时间：2026-01-09

- CA916798 gene is involved in cisplatin resistance in human lung cancer through PI3K/AKT pathway
- Journal of Army Medical University Vol. 35, Issue 7, Pages: 618-621(2013)
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  CLC： R73-361;R734.2;R979.11
- Published：2013
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祝冰晶,王宇亮,罗虎,周向东.CA916798基因通过PI3K/AKT通路参与肺癌顺铂耐药[J].陆军军医大学学报(原第三军医大学学报),2013,35(07):618-621.

　Zhu Bingjing,Wang Yuliang,Luo Hu,Zhou Xiangdong.CA916798 gene is involved in cisplatin resistance in human lung cancer through PI3K/AKT pathway[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(07):618-621.
祝冰晶,王宇亮,罗虎,周向东.CA916798基因通过PI3K/AKT通路参与肺癌顺铂耐药[J].陆军军医大学学报(原第三军医大学学报),2013,35(07):618-621. DOI：

　Zhu Bingjing,Wang Yuliang,Luo Hu,Zhou Xiangdong.CA916798 gene is involved in cisplatin resistance in human lung cancer through PI3K/AKT pathway[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(07):618-621. DOI：

摘要

目的在前期体外研究的基础上，建立裸鼠移植瘤模型，观察阻断PI3K/AKT通路对肺腺癌细胞A549及多药耐药肺腺癌细胞A549/CDDP内CA916798基因mRNA表达的影响。方法建立A549、A549/CDDP裸鼠移植瘤模型，以LY294002作用A549组和A549/CDDP组后，比较各组移植瘤生长情况，HE染色观察组织结构变化，实时荧光定量PCR检测肿瘤细胞中CA916798基因mRNA 的表达水平。结果成功建立裸鼠移植瘤模型，以LY294002分别阻断A549、A549/CDDP组裸鼠移植瘤细胞的PI3K/AKT通路后，移植瘤体积明显缩小，CA916798基因的表达明显下调（P0.05）。结论抑制PI3K/AKT通路能够明显抑制肺腺癌细胞A549及A549/CDDP的恶性增殖。

Abstract

Objective To determine the effect of blocking the PI3K/AKT pathway on the expression of CA916798 gene in nude mouse transplanted tumor of human lung adenocarcinoma cell line A549 and the multidrug-resistant cell line A549/CDDP. Methods A total of 20 nude mice were randomly and equally divided into 4 groups

that is

A549

A549/CDDP

A549+LY294002

and A549/CDDP+LY294002 groups. The animal model with A549 cells and A549/CDDP cells transplanted tumor was established in these corresponding nude mice. LY294002 of 25 mg/kg was treated in the mice of the 2 later groups. The size of tumor was measured

and morphology of the mass was observed after HE staining. Real-time fluorescent quantitative PCR was performed to detect CA916798 mRNA expression in the tumor. Results The model of transplanted tumor was established in nude mice successfully. Blocking PI3K/AKT pathway with LY294002 resulted in significantly decreased expression of CA916798 at mRNA level (P0.05)

and reduced tumor volume. Conclusion Inhibition of PI3K/AKT pathway significantly inhibits the malignant proliferation in lung adenocarcinoma cell A549 and multidrug-resistant cell line A549/CDDP.

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Related Institution

Department of Ophthalmology， First Affiliated Hospital， Army Medical University （Third Military Medical University）

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Department of Gastroenterology， Second Affiliated Hospital， Army Medical University （Third Military Medical University）

Department of Thoracic Surgery, Institute of Surgery Research, Daping Hospital, Third Military Medical University

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