Objective　　　 To determine the differences of human immortal keratinocyte line HaCaT with or without lentivius-mediated CCL20 over-expression by proteomic analysis in order to investigate the cause of altered growth characteristics and potential threat of tumorigenicity in the seed cells for gene-modified tissue engineering.　　　 Methods　　　 Lentiviral vector (pHSER-CCL20-shRNA-GFP)-infected HaCaT cells with significant changes in growth characteristics and uninfected HaCaT cells were chosen as experimental and control cells

respectively. The differentially expressed proteins between them were revealed by two-dimensional electrophoresis

and these obtained proteins were further identified by LC-MS-MS. Lamin B1

which is closely related to tumorigenicity

cell growth and cell cycle

was picked out from these proteins and its differential expressions at protein and mRNA levels were validated by Western blotting and quantitative PCR (qPCR).　　　 Results　　　 Between these 2 cell lines

there were 11 differentially expressed proteins found by two-dimensional electrophoresis

and 7 proteins including lamin B1 identified from them by LC-MS-MS. Furthermore

increased expression of lamin B1 protein in lentiviral vector-infected HaCaT cells was proved by Western blotting as well as its mRNA expression by qPCR analysis.　　　 Conclusion　　　 An over-expression of lamin B1 protein is seen in lentiviral vector-infected human immortal keratinocyte

which may be correlated with altered growth characteristics of lentiviral vector-infected keratinocyte.