Interferon-α/β inhibits lymphoma cell proliferation by up-regulating SARI expression

黄英辉; 张立; 黄艳; 黄刚; 杨朝辉; 何谐; 钟丹; 何凤田

您当前的位置：

首页 >

文章列表页 >

Interferon-α/β inhibits lymphoma cell proliferation by up-regulating SARI expression

更新时间：2026-01-09

- Interferon-α/β inhibits lymphoma cell proliferation by up-regulating SARI expression
- Journal of Army Medical University Vol. 34, Issue 24, Pages: 2465-2468(2012)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R73-362;R733.4;R977
- Published：2012
- 稿件说明：
移动端阅览
黄英辉,张立,黄艳,黄刚,杨朝辉,何谐,钟丹,何凤田.α/β干扰素通过上调SARI表达抑制淋巴瘤细胞的增殖[J].陆军军医大学学报(原第三军医大学学报),2012,34(24):2465-2468.

　Huang Yinghui,Zhang Li,Huang Yan,Huang Gang,Yang Chaohui,He Xie,Zhong Dan,He Fengtian.Interferon-α/β inhibits lymphoma cell proliferation by up-regulating SARI expression[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(24):2465-2468.
黄英辉,张立,黄艳,黄刚,杨朝辉,何谐,钟丹,何凤田.α/β干扰素通过上调SARI表达抑制淋巴瘤细胞的增殖[J].陆军军医大学学报(原第三军医大学学报),2012,34(24):2465-2468. DOI：

　Huang Yinghui,Zhang Li,Huang Yan,Huang Gang,Yang Chaohui,He Xie,Zhong Dan,He Fengtian.Interferon-α/β inhibits lymphoma cell proliferation by up-regulating SARI expression[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(24):2465-2468. DOI：

摘要

目的初步探讨α/β干扰素抑制淋巴瘤细胞增殖的分子机制。方法用IFN-α/β处理淋巴瘤细胞（Namalwa、JeKo-1）和白血病细胞（Jurkat、THP-1）24 h，CCK-8法检测IFN-α/β对上述细胞的增殖抑制效果；RT-PCR、real-time PCR、Western blot检测其SARI mRNA和蛋白水平的变化；对SARI基因启动子区的转录因子结合位点进行生物信息学预测。结果 IFN-α和IFN-β均可有效杀伤Namalwa和JeKo-1淋巴瘤细胞（P0.05），且均能显著诱导淋巴瘤细胞SARI mRNA和蛋白的表达（P0.05），但两种干扰素对Jurkat和THP-1白血病细胞的存活及SARI的表达均无显著影响（P0.05）；生物信息学分析显示，SARI启动子区含有转录因子STAT的结合位点。结论上调SARI表达可能是IFN-α/β抑制淋巴瘤细胞增殖的机制之一，而SARI的上调可能是由STAT介导的。

Abstract

Objective To investigate the mechanism of interferon-α/β (IFN-α/β) inhibiting lymphoma cell proliferation. Methods Lymphoma cells (Namalwa and JeKo-1) and leukemia cells (Jurkat and THP-1) were treated with IFN-α/β for 24 h. Cell viability was measured by CCK-8 assay. The mRNA and protein expression levels of SARI were detected by reverse transcription PCR

real-time PCR and Western blotting. The transcription factor binding sites in SARI gene promoter region were predicted using bioinformatics method. Results Lymphoma cells (Namalwa and JeKo-1) were effectively killed by both IFN-α and IFN-β (P0.05)

and the mRNA and protein expression levels of SARI were significantly up-regulated (P0.05). However

IFN-α/β had no significant effect on cell viability and SARI expression of leukemia cells (Jurkat and THP-1) (P0.05). Bioinformatics results revealed that transcription factor STAT might bind to SARI gene promoter region. Conclusion Upregulation of SARI

which is possibly mediated by STAT

may be involved in the process of IFN-α/β inhibiting lymphoma cell proliferation.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Efficacy and clinical outcomes of different antiviral regimens in HBeAg/HBeAb double-positive patients with chronic hepatitis B

Clinical application of CAR-T cells in treatment of hematologic malignancies

Diagnostic value of thoracoscopy for pleural effusion caused by lymphoma

Effect of interferons on expression of adenosine deaminase acting on RNA 1 and hepatitis B virus replication in human hepatocellular carcinoma cells in vitro

Value of ¹⁸F-FDG PET/CT in diagnosis of lymphoma and lymph node tuberculosis

Related Author

罗欢

胡鹏

向毅

巫贵成

夏莉娜

刘书宏

安选

黄瑞昊

Related Institution

No data

⁰