Effect of an ent-kaurane diterpene Wangzaozin A on proliferation and apoptosis in human promyelocytic leukemia HL-60 cells

丁兰; 第五佳丽; 田继东; 柳志军; 杨玲; 刘国安; 令利军

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Effect of an ent-kaurane diterpene Wangzaozin A on proliferation and apoptosis in human promyelocytic leukemia HL-60 cells

更新时间：2026-01-09

- Effect of an ent-kaurane diterpene Wangzaozin A on proliferation and apoptosis in human promyelocytic leukemia HL-60 cells
- Journal of Army Medical University Vol. 35, Issue 8, Pages: 727-732(2013)
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  CLC： R965;R733.7;R979.19
- Published：2013
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丁兰,第五佳丽,田继东,柳志军,杨玲,刘国安,令利军.对映-贝壳杉烷型二萜化合物Wangzaozin A对人早幼粒白血病HL-60细胞生长抑制和凋亡诱导的作用[J].陆军军医大学学报(原第三军医大学学报),2013,35(08):727-732.

　Ding Lan,Diwu Jiali,Tian Jidong,Liu Zhijun,Yang Ling,Liu Guoan,Ling Lijun.Effect of an ent-kaurane diterpene Wangzaozin A on proliferation and apoptosis in human promyelocytic leukemia HL-60 cells[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(08):727-732.
丁兰,第五佳丽,田继东,柳志军,杨玲,刘国安,令利军.对映-贝壳杉烷型二萜化合物Wangzaozin A对人早幼粒白血病HL-60细胞生长抑制和凋亡诱导的作用[J].陆军军医大学学报(原第三军医大学学报),2013,35(08):727-732. DOI：

　Ding Lan,Diwu Jiali,Tian Jidong,Liu Zhijun,Yang Ling,Liu Guoan,Ling Lijun.Effect of an ent-kaurane diterpene Wangzaozin A on proliferation and apoptosis in human promyelocytic leukemia HL-60 cells[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(08):727-732. DOI：

摘要

目的　　　研究对映-贝壳杉烷型二萜Wangzaozin A对人早幼粒白血病细胞HL-60生长抑制、周期阻滞、凋亡诱导作用以及相关的机制。　　　方法　　　利用倒置显微镜观察和台盼蓝排染法检测了Wangzaozin A对HL-60细胞生长的影响；采用流式细胞术和吖啶橙/溴化乙锭（AO/EB）双染法检测化合物对细胞周期分布的影响以及诱导HL-60细胞凋亡的能力；进一步利用单细胞凝胶电泳法测试化合物对细胞DNA的损伤。　　　结果　　　1~4 μmol/L的Wangzaozin A对HL-60细胞具有较强的生长抑制作用，较高浓度（3 μmol/L和4 μmol/L）和较长处理时间（48~72 h）有较强的致死效应；1~2.5 μmol/L药物处理细胞24 h或48 h条件下细胞周期分布主要表现为G1期阻滞；4 μmol/L药物处理12~60 h条件下细胞周期分布主要表现为很强的S期阻滞；2~4 μmol/L药物作用24 h或48 h可诱导凋亡产生，其凋亡率呈显著水平（P0.01），相同条件下，药物对细胞DNA的损伤与对照相比差异有统计学意义（P0.05，P0.01）。　　　结论　　　该化合物对HL-60细胞DNA的损伤作用极可能是引起细胞生长抑制、周期阻滞及凋亡的直接原因。

Abstract

Objective　　　To investigate the effect of Wangzaozin A on the proliferation and apoptosis of human promyelocytic leukemia HL-60 cells.　　　 Methods　　　HL-60 cells were treated with Wangzaozin A at different concentrations for different times. Trypan blue exclusion was performed to detect viability of HL-60 cells under an inverted microscope. Cell cycle arrest and apoptosis of HL-60 cells were detected by flow cytometry and AO/EB staining. DNA damage in HL-60 cells was d

etected by comet assay.　　　 Results　　　Wangzaozin A strongly inhibited the growth of HL-60 cells at the concentration of 1-4 mol/L

and induced strong lethal effect to the cells at high concentrations (3 mol/L and 4 mol/L) for 48-72 h. Strong G

phase and S phase arrest could be induced by Wangzaozain A at 1-2.5 mol/L for 24 h or 48 h and at 4 mol/L for 12-60 h

respectively

and significant apoptosis of HL-60 cells was induced by Wangzaozain A at 2-4 mol/L (P0.01). At the same time

the DNA damage in the treatment group was significantly different from that of the control group (P0.05

P0.01).　　　 Conclusion　　　Wangzaozin A induces proliferation suppression

cell cycle arrest and apoptosis in HL-60 cells directly through DNA damage.

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