Objective　　　To study three-dimensional morphology and spatial location of human deep brain nuclei.　　　 Methods　　　Cross-sectional head images of Chinese Visible Human Ⅱ (CVH-2) were selected and adjusted to a certain angle to meet anterior commissure (AC) and posterior commissure (PC) axial plane. The locations and outlines of deep brain nuclei were tracked in transversal

sagittal and coronal images continuously

and then the cross-sectional images as well as related structures were segmented by image processing software. Surface rendering and volume rendering were used to reconstruct nuclei and related structures. Meanwhile

the anatomical data of deep brain nuclei

such as spatial range

coordinate value of gravity center and volume

were measured and calculated.　　　 Results　　　The true-color cross-sectional head images that paralleled to AC-PC axial plane were obtained

and the outlines of deep brain nuclei in cross-sectional head images were segmented. Three-dimensional visible models of nuclei in deep brain were reconstructed

and the anatomical data such as coordinates of the gravity center

coordinate ranges and nuclear volume were acquired.　　　 Conclusion　　　The reconstructed three-dimensional model based on CVH-2 not only displays the three-dimensional morphology and spatial relationship of human deep brain nuclei

but also provides the anatomical data of the nuclei.