Gene expression profiles of activated T lymphocyte with IBP deficient by oligonucleotide microarray

向莉; 李鹏; 张竹君; 杨明珍; 陈安; 胡川闽

您当前的位置：

首页 >

文章列表页 >

Gene expression profiles of activated T lymphocyte with IBP deficient by oligonucleotide microarray

更新时间：2026-01-09

- Gene expression profiles of activated T lymphocyte with IBP deficient by oligonucleotide microarray
- Journal of Army Medical University Vol. 35, Issue 2, Pages: 109-113(2013)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R392.12;R394-33;R394.3
- Published：2013
- 稿件说明：
移动端阅览
向莉,李鹏,张竹君,杨明珍,陈安,胡川闽.IBP表达抑制后活化T淋巴细胞基因表达谱变化分析[J].陆军军医大学学报(原第三军医大学学报),2013,35(02):109-113.

　Xiang Li,Li Peng,Zhang Zhujun,Yang Mingzhen,Chen An,Hu Chuanmin.Gene expression profiles of activated T lymphocyte with IBP deficient by oligonucleotide microarray[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(02):109-113.
向莉,李鹏,张竹君,杨明珍,陈安,胡川闽.IBP表达抑制后活化T淋巴细胞基因表达谱变化分析[J].陆军军医大学学报(原第三军医大学学报),2013,35(02):109-113. DOI：

　Xiang Li,Li Peng,Zhang Zhujun,Yang Mingzhen,Chen An,Hu Chuanmin.Gene expression profiles of activated T lymphocyte with IBP deficient by oligonucleotide microarray[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(02):109-113. DOI：

摘要

目的利用全基因组寡核苷酸芯片检测干扰素调节因子-4结合蛋白（IRF-4 binding protein，IBP）表达抑制后活化T淋巴细胞基因表达谱的差异。方法采用本室构建的IBP表达抑制的Jurkat T细胞及相应对照细胞为实验对象，Anti-CD3、CD28 mAb处理细胞后提取刺激24、48 h的细胞总RNA，利用北京博奥生物有限公司22K Human Genome Array芯片检测基因表达的差异，以MAS.doc.V1软件，结合KEGG、NCBI等生物信息学数据库检索分析差异表达基因功能和网络关系。结果 IBP基因表达抑制的Jurkat T细胞在TCR信号刺激24、48 h后，细胞能量代谢、周期生长、转录调控及凋亡等多种类型的基因发生差异表达改变，2个时相组共有56个差异表达趋势一致基因，其中17个基因共同上调，39个基因共同下调。结论在活化的Jurkat T细胞中IBP表达抑制所致多个基因差异表达。

Abstract

Objective To investigate the difference of gene expression between activated IBP-deficient and wild T lymphocyte by oligonucleotide microarray in order to determine the role of IBP in T lymphocyte activation. Methods Total RNA was isolated from IBP-deficient and its parental Jurkat T cells with anti-CD3 and anti-CD28 mAb stimulation for 24 or 48 h

and synthesized into double-stranded cDNA that was then synthesized into biotin-labeled cRNA probe by in vitro transcription. The cRNA probes were separately hybridized with 22K Human Genome Array Chip

and the signals were scanned by the GeneArray Scanner. The results were analyzed by bioinformatics. Results In comparison with the expression profile of parental Jurkat T cells

anti-CD3 and anti-CD28 mAb stimulation for 24 or 48 h resulted in that 56 genes were found to have no change in the expression

17 genes were up-regulated and 39 genes down-regulated in activated IBP-deficient Jurkat T cells. According to Gene Ontology and Tree View analysis

these genes were involved in energy metabolism

cell cycle

transcription and apoptosis and so on. Conclusion IBP-deficient causes differential expression in many genes in Jurkat T cells.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Clinical characteristics of coronavirus disease 2019 in a Chongqing hospital: analysis of 89 cases

GeneChip array for screening ATF3 related genes associated with acute lung injury in mouse model

Rapid detection of SM/EMB/LVFX/AMK/CPM resistance by DNA microarray for clinical isolates from spinal tuberculosis

Correlation between expression of granzyme B and perforin and apoptosis in epithelial cells in oral lichen planus with local lymphocyte infiltration

Differential expression in retina of chronic high intraocular pressure SD rats by large-scale Oligo microarray

Related Author

高红丽

庞科

黄澄澄

吴学玲

郭亮

钱斓兰

周强

程鹏

Related Institution

No data

⁰