Role of miR-145 expression in arterial intimal hyperplasia of hypertension in rats

皮燕; 张莉莉; 胡子成; 黎炳护; 尹延伟; 高长越; 李敬诚

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Role of miR-145 expression in arterial intimal hyperplasia of hypertension in rats

更新时间：2026-01-09

- Role of miR-145 expression in arterial intimal hyperplasia of hypertension in rats
- Journal of Army Medical University Vol. 35, Issue 8, Pages: 707-711(2013)
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  CLC： R322.121;R394.2;R544.1
- Published：2013
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皮燕,张莉莉,胡子成,黎炳护,尹延伟,高长越,李敬诚.miR-145表达状态对大鼠高血压动脉内膜增生的影响[J].陆军军医大学学报(原第三军医大学学报),2013,35(08):707-711.

　Pi Yan,Zhang Lili,Hu Zicheng,Li Binghu,Yin Yanwei,Gao Changyue,Li Jingcheng.Role of miR-145 expression in arterial intimal hyperplasia of hypertension in rats[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(08):707-711.
皮燕,张莉莉,胡子成,黎炳护,尹延伟,高长越,李敬诚.miR-145表达状态对大鼠高血压动脉内膜增生的影响[J].陆军军医大学学报(原第三军医大学学报),2013,35(08):707-711. DOI：

　Pi Yan,Zhang Lili,Hu Zicheng,Li Binghu,Yin Yanwei,Gao Changyue,Li Jingcheng.Role of miR-145 expression in arterial intimal hyperplasia of hypertension in rats[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(08):707-711. DOI：

摘要

目的　　观察微小RNA-145(microRNA-145，miR-145)在高血压血管内膜增生中的作用。　　方法　　常规HE染色观察自发性高血压大鼠（SHR）和对照大鼠（WKY）主动脉内膜增生情况，逆转录多聚酶链反应（RT-PCR）方法检测不同病程SHR大鼠主动脉中miR-145的表达水平，并应用miR-145前体premiR-145和抑制剂2’OMe-miR-145分别升高和抑制血管平滑肌细胞（smooth muscle cell， VSMC）中miR-145的表达水平，从分化标志蛋白α平滑肌肌动蛋白（α-SMA）表达水平、VSMC的增殖和迁移能力等方面检测VSMC表型转化情况。　　结果　　 ①SHR大鼠主动脉内膜增生，内膜/中膜面积百分比（39.7±12.1）%显著高于WKY组（3.8±1.2）%（P=0.001），α-SMA染色阳性的VSMC是增生内膜的主要细胞成分；②与WKY相比，SHR大鼠主动脉来源的VSMC表型转化明显增多，表现为增殖和迁移能力升高，而α-SMA表达降低（P0.01）；③SHR大鼠主动脉中的miR-145水平在8周龄时开始下降（P=0.045 6），至18周龄时降至仅为WKY组的7.5%；④应用premiR-145可上调SHR-VSMC中α-SMA的表达（P=0.005 6）并促进VSMC增殖迁移过程（P0.01），而2’OMe-miR-145可抑制VSMC中α-SMA的表达（P=0.023 2）和VSMC增殖迁移过程（P0.01）。　　结论　　高血压时动脉中miR-145水平呈进行性下降，可能是引起VSMC表型转化并促进动脉内膜增生的一个重要机制。

Abstract

Objective　　 To determine the effect of miR-145 on arterial intimal hyperplasia in hypertension. 　　Methods　　 Spontaneous hypertension rats (SHR) and Wistar-Kyoto (WKY) rats were used in this study. Aortic intimal hyperplasia was evaluated pathologically. The expression of miR-145 in aorta of SHR was detected by reverse transcription polymerase chain reaction (RT-PCR). The phenotypic modulation of vascular smooth muscle cells (VSMCs) was assessed by detecting the expression of α-smooth muscle actin (α-SMA) and the capability of proliferation and migration in the presence of miR-145 precursor

premiR-145

or inhibitor 2’OMe-miR-145. 　　Results　　 SHR aortas exhibited obvious neo-intima formation which was composed primarily of VSMCs

with the ratios of intima/media significant higher than WKY rats (P=0.001). SHR-derived VSMCs exhibited marked phenotypic modulation

however

the expression of α-SMA was lower (P0.01). The expression of miR-145 in SHR aortas was declined at the 8th week (P=0.045 6) and reduced to 7.5% of that of WKY rats at the 18th week. Expression of α-SMA and proliferative and migratory capacities of VSMCs were respectively upregulated and downregulated by premiR-145 and 2’OMe-miR-145. 　　Conclusion　　 The expression of miR-145 decreases progressively during hypertension

which may at least partly contribute to the excessive VSMCs phenotypic modulation and thus arterial intimal hyperplasia.

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