Neuroprotective effect of liver X receptor agonist TO901317 on ganglion cells in alcohol-induced retinal injury in neonatal mice

唐永萍; 杨阳; 徐培; 徐海伟; 阴正勤

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Neuroprotective effect of liver X receptor agonist TO901317 on ganglion cells in alcohol-induced retinal injury in neonatal mice

更新时间：2026-01-09

- Neuroprotective effect of liver X receptor agonist TO901317 on ganglion cells in alcohol-induced retinal injury in neonatal mice
- Journal of Army Medical University Vol. 35, Issue 5, Pages: 376-380(2013)
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  CLC： R394.2;R595.6;R774.1
- Published：2013
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唐永萍,杨阳,徐培,徐海伟,阴正勤.TO901317修复酒精致新生小鼠视网膜节细胞损伤的实验研究[J].陆军军医大学学报(原第三军医大学学报),2013,35(05):376-380.

　Tang Yongping,Yang Yang,Xu Pei,Xu Haiwei,Yin Zhengqin.Neuroprotective effect of liver X receptor agonist TO901317 on ganglion cells in alcohol-induced retinal injury in neonatal mice[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(05):376-380.
唐永萍,杨阳,徐培,徐海伟,阴正勤.TO901317修复酒精致新生小鼠视网膜节细胞损伤的实验研究[J].陆军军医大学学报(原第三军医大学学报),2013,35(05):376-380. DOI：

　Tang Yongping,Yang Yang,Xu Pei,Xu Haiwei,Yin Zhengqin.Neuroprotective effect of liver X receptor agonist TO901317 on ganglion cells in alcohol-induced retinal injury in neonatal mice[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(05):376-380. DOI：

摘要

目的观察酒精暴露对新生小鼠视网膜结构的影响，以及肝脏X受体(LXRs)激动剂TO901317对酒精损伤小鼠视网膜的保护作用。方法将新生C57BL/6J小鼠分为对照组、酒精暴露组和激动剂TO预处理组，每组3只。采用HE染色检测各组小鼠视网膜大体结构改变，采用免疫组化染色检测各组小鼠视网膜NeuN阳性细胞数、GFAP表达变化。结果免疫组化染色发现LXRβ主要分布于新生小鼠视网膜节细胞层，HE染色发现酒精组新生小鼠视网膜节细胞数量较对照组显著减少（P0.01），TO处理能显著翻转酒精暴露导致的视网膜节细胞层细胞数量减少（P0.05）；免疫组化染色发现酒精组小鼠眼球视网膜节细胞层NeuN阳性细胞数明显少于对照组（P0.01），而TO预处理组较酒精组NeuN阳性细胞显著增多（P0.01）。对各组进行GFAP免疫组化染色并采用光密度分析进行半定量分析，结果显示酒精组小鼠视网膜GFAP表达较对照组和TO预处理组显著增高（P0.01）。结论 LXRs受体激活可有效抑制酒精所致视网膜中星形胶质细胞的活化，挽救节细胞丢失。

Abstract

Objective To observe the effect of alcohol exposure on the retina of neonatal mice and the neuroprotective role of liver X receptor (LXR) agonist TO901317 (TO). Methods Mice were randomly divided into 3 groups

including a control group

an alcohol exposure group and a TO pretreatment group (n=3). Hematoxylin and eosin (HE) staining was used to observe the general structure of mouse retina

and immunochemistry was applied to detect NeuN-positive neurons and GFAP expression in the retina. Results LXRβ was mainly expressed in retinal ganglion cell layer of neonatal (postnatal day 6

P6) mice. HE staining showed a significant decrease in cell number in retinal ganglion cell layer of the alcohol exposure group compared with the control group (P0.01)

while TO could protect the ganglion cells from alcohol damage (P0.05). Compared with the control group

NeuN-positive cells in the retinal ganglion cell layer of the alcohol exposure group significantly decreased (P0.01)

but the TO pretreatment group had more NeuN-positive cells than the alcohol exposure group (P0.01). GFAP expression significantly increased in the retinal ganglion cell layer of the alcohol exposure group as compared with the control group and TO pretreatment group (P0.01). Conclusion LXR agonist TO can inhibit alcohol-induced astrocyte activation in retina and rescue retinal ganglion cells in neonatal mice.

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