Molecular typing and antimicrobial susceptibility of methicillin-resistant Staphylococcus aureus isolated from Guangzhou, China

程航; 曾方银; 胡启文; 袁文常; 周人杰; 张霞; 袁吉振; 尚伟龙; 杨杰; 胡珍; 朱军民; 饶贤才

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Molecular typing and antimicrobial susceptibility of methicillin-resistant Staphylococcus aureus isolated from Guangzhou, China

更新时间：2026-01-09

- Molecular typing and antimicrobial susceptibility of methicillin-resistant Staphylococcus aureus isolated from Guangzhou, China
- Journal of Army Medical University Vol. 35, Issue 8, Pages: 696-701(2013)
- 作者机构：
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  CLC： R181.34;R372;R378.11
- Published：2013
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程航,曾方银,胡启文,袁文常,周人杰,张霞,袁吉振,尚伟龙,杨杰,胡珍,朱军民,饶贤才.广州地区耐甲氧西林金黄色葡萄球菌的分子分型及耐药性分析[J].陆军军医大学学报(原第三军医大学学报),2013,35(08):696-701.

　Cheng Hang,Zeng Fangyin,Hu Qiwen,Yuan Wenchang,Zhou Renjie,Zhang Xia,Yuan Jizhen,Shang Weilong,Yang Jie,Hu Zhen,Zhu Junmin,Rao Xiancai.Molecular typing and antimicrobial susceptibility of methicillin-resistant Staphylococcus aureus isolated from Guangzhou, China[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(08):696-701.
程航,曾方银,胡启文,袁文常,周人杰,张霞,袁吉振,尚伟龙,杨杰,胡珍,朱军民,饶贤才.广州地区耐甲氧西林金黄色葡萄球菌的分子分型及耐药性分析[J].陆军军医大学学报(原第三军医大学学报),2013,35(08):696-701. DOI：

　Cheng Hang,Zeng Fangyin,Hu Qiwen,Yuan Wenchang,Zhou Renjie,Zhang Xia,Yuan Jizhen,Shang Weilong,Yang Jie,Hu Zhen,Zhu Junmin,Rao Xiancai.Molecular typing and antimicrobial susceptibility of methicillin-resistant Staphylococcus aureus isolated from Guangzhou, China[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(08):696-701. DOI：

摘要

目的　　了解广州地区耐甲氧西林金黄色葡萄球菌(methicillin-resistant Staphylococcus aureus，MRSA)分子型别及其对临床常用抗生素的耐药情况，为防控MRSA感染提供依据。　　方法　　收集2010年10月至2011年4月广州地区两家教学医院临床分离的MRSA 142株，应用SCCmec、spa、多位点序列分型(multilocus sequence typing，MLST)、脉冲场凝胶电泳(pulsed-field gel electrophoresis，PFGE)等方法进行分子分型，检测这些菌株对苯唑西林、克林霉素等17种临床常用抗生素的药物敏感性，分析临床MRSA的耐药情况。　　结果　　 142株MRSA中121株为医院获得型金葡菌（HA-MRSA），20株为社区获得型金葡菌（CA-MRSA），1株未定型MRSA。这些MRSA可分为16种spa型，8种ST型及13种PFGE型。HA-MRSA中的ST239-MRSA-Ⅲ-t030、ST239-MRSA-Ⅲ-t037和ST5-MRSA-Ⅱ-t002为主要流行克隆，也发现CA-MRSA 中ST59-MRSA-Ⅳ-t437克隆的流行，且这些流行克隆有特定的耐药谱。　　结论　　广州地区流行的金葡菌仍以HA-MRSA为主，同时也有一定量的CA-MRSA检出和传播，且CA-MRSA的多重药物敏感性已在下降。

Abstract

Objective　　 To determine the molecular types and drug susceptibilities of methicillin-resistant Staphylococcus aureus (MRSA) prevailed in Guangzhou

China. 　　Methods 　　A total of 142 MRSA isolates were collected between October 2010 and April 2011 from two teaching hospitals in Guangzhou. The molecular typing methods

such as staphylococcal cassette chromosome mec (SCCmec) typing

staphylococcal protein A (spa) typing

multilocus sequence typing (MLST)

and pulsed-field gel electrophoresis (PFGE)

were applied for all MRSA strains

and the drug susceptibilities to 17 kinds of antibiotics were also determined. 　　Results　　 In the 142 MRSA isolates

there were 121 hospital-associated isolates (HA-MRSA)

20 community-associated isolates (CA-MRSA)

and 1 non typeable MRSA. Sixteen spa types

eight sequence types (ST)

and thirteen PFGE types were found. The major predominant clones were ST239-MRSA-Ⅲ-t030

ST239-MRSA-Ⅲ-t037 and ST5-MRSA-Ⅱ-t002 that belong to HA-MRSA. Furthermore

colne ST59-MRSA-Ⅳ-t437 that belongs to CA-MRSA was also found to be prevailed in Guangzhou. The major prevailed clones with specific drug resistant profiles were found. 　　Conclusion　　 HA-MRSA is still the most predominant MRSA in Guangzhou

and CA-MRSA with decreasing drug susceptibility is also found. It is the time to monitor the prevalence of CA-MRSA and drug resistance of MRSA.

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