Objective      To determine the role of CIP2A in the development and progression of laryngeal squamous cell carcinoma and investigate its clinical significance.       Methods      Expression of CIP2A was examined with immunohistochemistry in 61 tissue samples of pathologically identified laryngeal squamous cell carcinoma and their 40 adjacent normal tissues

9 atypical hyperplasia and 8 laryngeal papilloma. The relationship between CIP2A with the clinicalpathological characteristics and the effect of CIP2A expression on prognosis were analyzed.       Results      The expression of CIP2A was significantly higher in laryngeal squamous cell carcinoma than atypical hyperplasia and laryngeal papilloma (P0.05). CIP2A immunoreactivity

tested in 61 laryngeal cancer tissues

was negative in 11 cases (18.0%)

weakly positive in 13 cases (21.3%)

moderately positive in 25 cases (41.0%)

and strongly positive in 12 cases (19.7%). The expression of CIP2A was not related with age

sex

smoking and alcohol assumption. Whereas

there were significant correlations of CIP2A with T stage

clinical stage

lymph node metastasis

and histological differentiation (P0.05). High CIP2A expression predicted poor survival

and was associated with shorter overall-survival and disease-free survival.       Conclusion      Over-expression of CIP2A might be involved in the development and progression of laryngeal squamous cell carcinoma. CIP2A may serve as a useful prognostic marker and therapeutic target for laryngeal squamous cell carcinoma.