Effect of propranolol on expression of MMP-2, MMP-9, TIMP-1 and TIMP-2 in infantile hemangioma

李铭; 陈幸生; 郭志辉; 谢义德; 詹明坤; 周亚宽; 江成鸿; 陈小松

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Effect of propranolol on expression of MMP-2, MMP-9, TIMP-1 and TIMP-2 in infantile hemangioma

更新时间：2026-01-09

- Effect of propranolol on expression of MMP-2, MMP-9, TIMP-1 and TIMP-2 in infantile hemangioma
- Journal of Army Medical University Vol. 34, Issue 23, Pages: 2403-2407(2012)
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  CLC： R732.2;R966;R972
- Published：2012
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李铭,陈幸生,郭志辉,谢义德,詹明坤,周亚宽,江成鸿,陈小松.口服普萘洛尔对婴幼儿血管瘤MMP-2、MMP-9、TIMP-1、TIMP-2表达水平的影响[J].陆军军医大学学报(原第三军医大学学报),2012,34(23):2403-2407.

　Li Ming,Chen Xingsheng,Guo Zhihui,Xie Yide,Zhan Mingkun,Zhou Yakuan,Jiang Chenghong,Chen Xiaosong.Effect of propranolol on expression of MMP-2, MMP-9, TIMP-1 and TIMP-2 in infantile hemangioma[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(23):2403-2407.
李铭,陈幸生,郭志辉,谢义德,詹明坤,周亚宽,江成鸿,陈小松.口服普萘洛尔对婴幼儿血管瘤MMP-2、MMP-9、TIMP-1、TIMP-2表达水平的影响[J].陆军军医大学学报(原第三军医大学学报),2012,34(23):2403-2407. DOI：

　Li Ming,Chen Xingsheng,Guo Zhihui,Xie Yide,Zhan Mingkun,Zhou Yakuan,Jiang Chenghong,Chen Xiaosong.Effect of propranolol on expression of MMP-2, MMP-9, TIMP-1 and TIMP-2 in infantile hemangioma[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(23):2403-2407. DOI：

摘要

目的研究口服普萘洛尔前后婴幼儿血管瘤病灶内MMP-2、MMP-9、TIMP-1、TIMP-2表达水平的影响。 方法 选择2010年5月至2011年12月来我院就诊、年龄≤3个月、血管瘤病灶位于肢体或隐蔽部位、家属要求手术治疗却具备单独口服普萘洛尔治疗条件、排除口服普萘洛尔禁忌证、先前未接受过其他任何相关治疗的病例作为研究对象，共39例。经过与家属沟通并征得其书面同意，先按口服普萘洛尔诊疗常规，口服用药前在局麻下夹取血管瘤体组织活检，后口服8周普萘洛尔[剂量：2 mg/(kg·d)，分2次，每12小时用药]，继而手术切除瘤体。采用免疫组化、实时荧光定量RT-PCR 的方法，检测口服用药前后病灶组织内MMP-2、MMP-9、TIMP-1、TIMP-2表达水平的变化，计算口服用药前后病灶微血管密度（MVD）。结果免疫组化与实时荧光定量RT-PCR结果均提示，口服普萘洛尔后血管瘤病灶内MVD值明显减低且具有统计学意义（t=18.458

P0.01）

MMP-2、MMP-9表达水平较用药前明显减少（P0.01），TIMP-1、TIMP-2表达水平明显增高（P0.01）。且口服用药后上述4个因子免疫组化平均积分光密度、mRNA相对表达量变化与MVD值变化密切相关。结论普萘洛尔可下调血管瘤病灶内MMP-2、MMP-9的表达，上调TIMP-1、TIMP-2的表达，从而抑制新生血管的形成。

Abstract

Objective To investigate the effect of propranolol on the expression of matrix metalloproteinase 2 (MMP-2)

matrix metalloproteinase 9 (MMP-9)

tissue inhibitor of metalloproteinase 1 (TIMP-1) and tissue inhibitor of metalloproteinase 2 (TIMP-2) in infantile hemangioma. Methods Thirty-nine patients (age≤3 months) with proliferative phase of hemangioma in our hospital from May

2010 to December

2010 were enrolled. The patients were treated with propranolol for 8 weeks after hemangioma biopsy

and then underwent surgery for hemangioma resection. The mRNA and protein expression levels of MMP-2

MMP-9

TIMP-1 and TIMP-2 in hemangioma tissues were examined by quantitative real-time RT-PCR and immunohistochemical method

respectively

and the microvessel density (MVD) was calculated before and after propranolol treatment. Results The MVD in the hemangioma tissues decreased significantly after propranolol treatment (P0.01). The protein and mRNA expressions of MMP-2 and MMP-9 decreased significantly (P0.01)

but the protein and mRNA expressions of TIMP-1 and TIMP-2 increased significantly after propranolol treatment (P0.01). The changes of MVD were closely correlated with the protein and mRNA expression levels of MMP-2

MMP-9

TIMP-1 and TIMP-2. Conclusion Propranolol can reduce proliferative hemangioma through down-regulating MMP-2 and MMP-9 expression and up-regulating TIMP-1 and TIMP-2 expression.

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