Effect of albendazole on glycolysis of cisplatin-resistant ovarian cancer cells

何迎盈; 刘娜娜; 罗治彬; 李少林

您当前的位置：

首页 >

文章列表页 >

Effect of albendazole on glycolysis of cisplatin-resistant ovarian cancer cells

更新时间：2026-01-09

- Effect of albendazole on glycolysis of cisplatin-resistant ovarian cancer cells
- Journal of Army Medical University Vol. 34, Issue 14, Pages: 1426-1429(2012)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： Q493.4;R737.31;R978.6
- Published：2012
- 稿件说明：
移动端阅览
何迎盈,刘娜娜,罗治彬,李少林.阿苯达唑对耐药卵巢癌细胞糖酵解的影响[J].陆军军医大学学报(原第三军医大学学报),2012,34(14):1426-1429.

　He Yingying,Liu Nana,Luo Zhibin,Li Shaolin.Effect of albendazole on glycolysis of cisplatin-resistant ovarian cancer cells[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(14):1426-1429.
何迎盈,刘娜娜,罗治彬,李少林.阿苯达唑对耐药卵巢癌细胞糖酵解的影响[J].陆军军医大学学报(原第三军医大学学报),2012,34(14):1426-1429. DOI：

　He Yingying,Liu Nana,Luo Zhibin,Li Shaolin.Effect of albendazole on glycolysis of cisplatin-resistant ovarian cancer cells[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(14):1426-1429. DOI：

摘要

目的探讨阿苯达唑（albendazole，ABZ）对人耐顺铂卵巢癌SKOV-3/DDP细胞糖酵解的影响。方法通过溴化二甲噻唑二苯四氨唑（MTT）比色法检测ABZ对卵巢癌SKOV-3/DDP细胞体外增殖的影响，求得抑制率分别为0%、25%、50%、75%时所对应的ABZ的浓度，实验分为对照组、IC

组、IC

组和IC

组，每组给予相应浓度的ABZ处理，并且按照ABZ作用时间再将每组分为12、24、36 h亚组。按照分组，在设定的时间点，分别用比色法测定己糖激酶（hexokinase，HK）和丙酮酸激酶（pyruvate kinase，PK）活性，用酶标仪法测定乳酸脱氢酶（lactate dehydrogenase，LDH）的活性，用RT-PCR法测定Akt mRNA和Myc mRNA的表达。结果经过不同浓度的ABZ处理SKOV-3/DDP细胞发现，ABZ能抑制SKOV-3/DDP细胞体外生长（P0.05），呈剂量依赖性，抑制率为0%所对应的ABZ浓度为0 μmol/L，抑制率25%的浓度（IC

）为（1.43±0.21）μmol/L，抑制率50%的浓度（IC

）为（8.64±0.74）μmol/L，抑制率75%的浓度（IC

）为（52.48±3.99）μmol/L。与对照组相比，经过ABZ处理的SKOV-3/DDP细胞的HK、PK、LDH活性明显降低（P0.05），且Akt 基因和Myc基因的表达下调（P0.05）。结论 ABZ能够抑制SKOV-3/DDP细胞糖酵解酶活性，并能下调糖酵解相关基因的表达。

Abstract

Objective To study the effect of albendazole (ABZ) on glycolysis of human cisplatin-resistant ovarian cancer SKOV3/DDP cells. Methods Proliferation of SKOV3/DDP cells was detected by methyl thiazolyl tetrazolium (MTT) colorimetry after treatment with ABZ in vitro. SKOV3/DDP cells were divided into control group

roup

group

and IC

group according to the concentration of ABZ with an inhibition rate of 0%

25%

50%

and 75%. Each group was further divided into 12

24 and 36 h subgroups according to the action time of ABZ at a certain concentration. Activities of hexokinase (HK)

pyruvate kinase (PK) and lactate dehydrogenase (LDH) were detected by colorimetry and ELISA

respectively

and expression of Akt mRNA and Myc mRNA was detected by RT-PCR at the setting time points. Results ABZ inhibited the proliferation of SKOV3/DDP cells in a dose-dependent manner (P0.05) with an inhibition rate of 0%

25%

50% and 75% at the concentration of 0

1.43±0.21

8.64±0.74 and 52.48±3.99 μmol/L

respectively

in control group

group

group and IC75 group. The activities of LDH

PK and the expression levels of Akt mRNA and Myc mRNA were significantly lower in IC

group

group and IC

group than in control group（P0.05）. Conclusion ABZ can inhibit the activities of glycolytic enzymes and down-regulate the expression of glycolysis-related genes in vitro.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Dihydromyricetin ameliorates myocardial glucose absorption and glycolysis in mice with diabetic cardiomyopathy

Role of pyruvate kinase M20-regulated glycolysis in inflammatory activation of macrophages in diabetic nephropathy

Down-regulation of integrin-linked kinase suppresses glycolysis and proliferation and promotes apoptosis of cervical cancer cells possibly by inhibiting Wnt/β-catenin pathway

Effect of 3-bromopyruvate combined with cisplatin on inhibiting growth of lung cancer A549 cells

Homo sapiensv-Ki-ras2 Kirsten rat sarcoma viral oncogenes homologue promotes glycolysis in lung cancer cells

Related Author

糜漫天

易龙

汪小兰

蒋玲

武璐婷

岳静

候鹏飞

董妞

Related Institution

Department of Oncology, Xinqiao Hospital, Third Military Medical University

Second Department of Respiratory Medicine, First Affiliated Hospital of Luzhou Medical College

⁰