Effect of thiostrepton on proliferation and FoxM1 expression in human laryngocarcinoma Hep-2 cell line

林力; 邓碧; 寿铸; 梁佳; 陈鸿雁

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Effect of thiostrepton on proliferation and FoxM1 expression in human laryngocarcinoma Hep-2 cell line

更新时间：2026-01-09

- Effect of thiostrepton on proliferation and FoxM1 expression in human laryngocarcinoma Hep-2 cell line
- Journal of Army Medical University Vol. 34, Issue 20, Pages: 2086-2089(2012)
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  CLC： R730.23;R739.65;R978.19
- Published：2012
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林力,邓碧,寿铸,梁佳,陈鸿雁.硫链丝菌肽对人喉癌Hep-2细胞生长及FoxM1表达的影响[J].陆军军医大学学报(原第三军医大学学报),2012,34(20):2086-2089.

　Lin Li,Deng Bi,Shou Zhu,Liang Jia,Chen Hongyan.Effect of thiostrepton on proliferation and FoxM1 expression in human laryngocarcinoma Hep-2 cell line[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(20):2086-2089.
林力,邓碧,寿铸,梁佳,陈鸿雁.硫链丝菌肽对人喉癌Hep-2细胞生长及FoxM1表达的影响[J].陆军军医大学学报(原第三军医大学学报),2012,34(20):2086-2089. DOI：

　Lin Li,Deng Bi,Shou Zhu,Liang Jia,Chen Hongyan.Effect of thiostrepton on proliferation and FoxM1 expression in human laryngocarcinoma Hep-2 cell line[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(20):2086-2089. DOI：

摘要

目的研究硫链丝菌肽(thiostrepton，TST)对人喉癌Hep-2细胞株增殖能力及FoxM1表达的影响。方法用MTT法测定TST作用于人喉癌Hep-2细胞48 h的增殖抑制率；通过流式细胞仪（FCM）检测不同浓度TST分别作用于Hep-2细胞48 h后对其细胞周期动力学的影响；采用免疫细胞化学法、RT-PCR和Western blot检测Hep-2细胞中FoxM1 mRNA及蛋白表达，以及不同浓度TST作用于Hep-2细胞48 h FoxM1蛋白表达的变化。结果不同浓度（1、2、4、8、12、16 μmol/L）TST作用于Hep-2细胞48 h后的增殖抑制率分别为5.27%、9.43%、20.25%、41.61%、67.96%、96.95%；浓度分别为2、3 μmol/L的TST作用于Hep-2细胞48 h后，均使Hep-2细胞停滞于G

期(P0.05)，且呈剂量依赖性(P0.05)；同时将TST作用于Hep-2细胞48 h后，经免疫细胞化学法、RT-PCR和Western blot均检测到FoxM1 mRNA 及蛋白在Hep-2细胞中表达显著降低(P0.05)。结论 TST对人喉癌Hep-2细胞株具有明显的增殖抑制作用，其可能机制是与降低肿瘤细胞中FoxM1蛋白表达有关。

Abstract

Objective To study the effect of thiostrepton (TST) on proliferation of and FoxM1 expression in human laryngocarcinoma Hep-2 cell line. Methods Proliferation inhibition rate of TST for human laryngocarcinoma Hep-2 cell line was measured by MTT reduction assay 48 h after it was used. Effect of TST at different concentrations on cell cycle of human laryngocarcinoma Hep-2 cells was assayed by flow cytometry. Expression of FoxM1 mRNA and protein in hum

an laryngocarcinoma Hep-2 cells and effect of TST at different concentrations were detected by immunocytochemistry

RT-PCR and Western blotting

respectively. Results The proliferation inhibition rate of TST at the concentrations of 1

12 and 16 μmol/L on proliferation of Hep-2 cells was 5.27%

9.43%

20.25%

41.61%

67.96% and 96.95%

respectively

in 48 h after treatment. TST at the concentrations of 2 μmol/L and 3 μmol/L arrested Hep-2 cells at the stage G

in a dose-dependent manner in 48 h after treatment (P0.05). Immunocytochemistry

RT-PCR and Western blotting showed that the expression levels of FoxM1 mRNA and protein were significantly lower in Hep-2 cells in 48 h after TST treatment (P0.05) . Conclusion TST significantly inhibits the proliferation of human laryngocarcinoma Hep-2 cell line

which is possibly with the down-regulated FoxM1 protein expression in the cells.

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