Role of connexin 43 in protecting rat myocardium against ischemia/reperfusion injury in vitro at sevoflurane post-conditioning

庞勇; 刘新伟; 何东伟; 刘柳

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Role of connexin 43 in protecting rat myocardium against ischemia/reperfusion injury in vitro at sevoflurane post-conditioning

更新时间：2026-01-09

- Role of connexin 43 in protecting rat myocardium against ischemia/reperfusion injury in vitro at sevoflurane post-conditioning
- Journal of Army Medical University Vol. 34, Issue 14, Pages: 1422-1425(2012)
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  CLC： R654.1;R965;R971.2
- Published：2012
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庞勇,刘新伟,何东伟,刘柳.缝隙连接蛋白43在七氟醚后处理保护大鼠离体心肌缺血再灌注中的作用[J].陆军军医大学学报(原第三军医大学学报),2012,34(14):1422-1425.

　Pang Yong,Liu Xinwei,He Dongwei,Liu Liu.Role of connexin 43 in protecting rat myocardium against ischemia/reperfusion injury in vitro at sevoflurane post-conditioning[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(14):1422-1425.
庞勇,刘新伟,何东伟,刘柳.缝隙连接蛋白43在七氟醚后处理保护大鼠离体心肌缺血再灌注中的作用[J].陆军军医大学学报(原第三军医大学学报),2012,34(14):1422-1425. DOI：

　Pang Yong,Liu Xinwei,He Dongwei,Liu Liu.Role of connexin 43 in protecting rat myocardium against ischemia/reperfusion injury in vitro at sevoflurane post-conditioning[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(14):1422-1425. DOI：

摘要

目的探讨缝隙连接蛋白（connexin，Cx）43在七氟醚后处理保护大鼠离体心肌缺血再灌注中的作用。方法健康成年雄性SD大鼠40只，体质量200~250 g，采用随机数字表法将其分为4组（n=10）：缺血再灌注组（IR组）、七氟醚后处理组（S组）、七氟醚混合5-羟基葵酸钠（5-HD）组（SH组）及5-HD组（H组）。采用Langendorff装置进行离体心脏灌注。各组均在阻断左冠状动脉（LAD）前维持灌注10 min，然后阻断LAD血流30 min后开放，再灌注120 min，其中S、SH及H组分别于LAD开放后使用3%七氟醚预饱和的K-H液、3%预饱和的七氟醚预充饱和的K-H加5-HD（100 μmol/L）及加入5-HD（100 μmol/L）的K-H液灌注15 min，然后使用普通K-H液继续灌注105 min。分别于阻断LAD前即刻（T0），开放LAD即刻（T1），开放LAD后15 min（T2），开放LAD后135 min（T3）时记录HR、左心室收缩压（LVSP）、左心室舒张压（LVDP）、左心室最大上升及下降速率（±dp/dt

max

）。再灌注结束后，取左心室TTC染色测定心肌梗死面积，采用免疫组化染色观测Cx43蛋白表达情况，采用Western blot法检测Cx43蛋白及磷酸化Cx43（p-Cx43）蛋白含量。结果与IR组比较，S组的HR、LVSP、±dp/dt

max

较高，LVDP降低，心肌梗死面积小，Cx43蛋白及p-Cx43蛋白含量增加，有统计学意义（P0.05）。IR组与SH组及H组之间相比HR、LVSP、±dp/dt

max

、LVDP、心肌梗死面积、Cx43蛋白和p-Cx43蛋白差异无显著性（P0.05）。结论七氟醚后处理减轻离体大鼠心肌IR损伤的作用可能与其开放线粒体敏感钾通道从而促进Cx43蛋白表达及其磷酸化有关。

Abstract

Objective To study the role of connexin43 (Cx43) in protecting rat myocardium against ischemia/reperfusion (I/R) injury in vitro at sevoflurane post-conditioning. Methods Forty healthy adul

t male SD rats

weighing 200-250 g

were randomly divided into I/R group

sevoflurane post-conditioning group (group S)

combined sevoflurane and 5-HD group (group SH)

and 5-HD group (group H)

10 in each group. In vitro heart was perfused with a Langendoff device for 10 min before the left coronary artery was occluded. The left coronary artery was reperfused after its blood flow was occluded for 30 min followed by 120 min-reperfusion. HR

LVSP

LVDP

and dp/dt

max

were recorded at occlusion of left coronary artery (T0)

intermediate reperfusion (T1)

15 min-reperfusion (T2) and 135 min-reperfusion (T3)

respectively. After reperfusion

myocardial infarct size was measured with TTC staining and expression level of Cx43 and its phosporylation protein p-Cx43 was measured with immunohistochemistry staining and Western blotting

respectively. Results The HR

LVSP and dp/dt

max

were higher

the LVDP was lower

the myocardial infarct size was smaller

and the expression level of Cx43 and its p-Cx43 was higher in group S than in I/R group (P0.05). However

no difference was found in the above parameters among I/R group

group SH and group H (P0.05). Conclusion The role of Cx43 in protecting rat myocardium against I/R injury in vitro at sevoflurane post-conditioning may be related with the open mito-KATP

and thus promote expression of Cx43 and its p-Cx43.

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