Expression of L amino acid transporter 1 and its heterodimer CD98hc in gastric cancer SGC-7901 cells and effect of the former on biological behaviors of the cells

史良会; 张才全; 黄士隋

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Expression of L amino acid transporter 1 and its heterodimer CD98hc in gastric cancer SGC-7901 cells and effect of the former on biological behaviors of the cells

更新时间：2026-01-09

- Expression of L amino acid transporter 1 and its heterodimer CD98hc in gastric cancer SGC-7901 cells and effect of the former on biological behaviors of the cells
- Journal of Army Medical University Vol. 34, Issue 14, Pages: 1418-1421(2012)
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  CLC： R394.2;R730.23;R735.2
- Published：2012
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史良会,张才全,黄士隋.LAT1及CD98hc在胃癌细胞SGC-7901中的表达及LAT1对其细胞生物学行为的影响[J].陆军军医大学学报(原第三军医大学学报),2012,34(14):1418-1421.

　Shi Lianghui,Zhang Caiquan,Huang Shisui.Expression of L amino acid transporter 1 and its heterodimer CD98hc in gastric cancer SGC-7901 cells and effect of the former on biological behaviors of the cells[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(14):1418-1421.
史良会,张才全,黄士隋.LAT1及CD98hc在胃癌细胞SGC-7901中的表达及LAT1对其细胞生物学行为的影响[J].陆军军医大学学报(原第三军医大学学报),2012,34(14):1418-1421. DOI：

　Shi Lianghui,Zhang Caiquan,Huang Shisui.Expression of L amino acid transporter 1 and its heterodimer CD98hc in gastric cancer SGC-7901 cells and effect of the former on biological behaviors of the cells[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(14):1418-1421. DOI：

摘要

目的观察L型氨基酸转运载体1（LAT1）与其异二聚体CD98hc在胃癌细胞SGC-7901中的表达及LAT1对细胞增殖与迁移能力的影响。方法将胃癌细胞SGC-7901分为1、10、100 μmol/L BCH 干预和空白对照共4组，RT-PCR、IF与Western blot检测胃癌细胞SGC-7901中LAT1与CD98hc mRNA、蛋白的表达。不同浓度的BCH作用于胃癌细胞SGC-7901 48 h后，新型四唑氮盐（MTS）比色法检测各组细胞生长情况，流式细胞仪检测各组细胞周期分布情况，Transwell 细胞迁移小室检测各组细胞迁移能力的变化。结果 LAT1及其异二聚体CD98hc在胃癌细胞SGC-7901中呈高表达。与空白对照组相比，随着BCH浓度的提高，胃癌细胞SGC-7901的增殖能力与迁移能力逐渐降低。细胞周期分析发现，BCH处理的胃癌细胞组S期细胞减少，而G

期细胞增加（P0.05）。结论 LAT1及其异二聚体CD98hc在SGC-7901细胞中有表达，LAT1可能促进SGC-7901细胞的增殖，加快细胞周期进程，增强SGC-7901细胞的迁移能力。

Abstract

Objective To observe the expression of L amino acid transporter 1 (LAT1) and its heterodimer CD98hc in gastric cancer SGC-7901 cells and the effect of LAT1 on proliferation and migration of SGC-7901 cells. Methods Gastric cancer SGC-7901 cells were divided into 1 μmol/L BCH intervention group，10 mol/L BCH intervention group，100 μmol/L BCH intervention group and control group. Expression of LAT1 and CD98hc mRNA in gastric cancer SGC-790

1 cells was detected by RT-PCR

IF and Western blotting

respectively. After the SGC-7901 cells were cultured with BCH at different concentrations for 48 h

their proliferation was detected by MTS colorimetry

distribution of cell cycle in different groups was assayed by flow cytometry

and cell migration in different groups was observed in Transwell chamber. Results LAT1 and CD98hc were highly expressed in SGC-7901 cells. The proliferation and migration level in SGC-7901 cells decreased with the increasing BCH concentration and were significantly lower in 3 BCH intervention groups than in control group. Cell cycle analysis showed that the number of SGC-7901 cells was lesser in S phase and greater in G

phase after BCH treatment（P0.05）. Conclusion LAT1 and CD98hc are expressed in SGC-7901 cells. LAT1 may promote the proliferation and migration of SGC-7901 cells and speed up their cell cycle process.

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