Protection of gentiopicroside on acute lung injury in sepsis mice

陈露露; 徐关丽; 袁红梅; 蔡江晖; 刘颖菊

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Protection of gentiopicroside on acute lung injury in sepsis mice

更新时间：2026-01-09

- Protection of gentiopicroside on acute lung injury in sepsis mice
- Journal of Army Medical University Vol. 34, Issue 23, Pages: 2392-2394(2012)
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  CLC： R285.5;R563;R631
- Published：2012
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陈露露,徐关丽,袁红梅,蔡江晖,刘颖菊.龙胆苦苷对脓毒症所致急性肺损伤小鼠的保护作用[J].陆军军医大学学报(原第三军医大学学报),2012,34(23):2392-2394.

　Chen Lulu,Xu Guanli,Yuan Hongmei,Cai Jianghui,Liu Yingju.Protection of gentiopicroside on acute lung injury in sepsis mice[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(23):2392-2394.
陈露露,徐关丽,袁红梅,蔡江晖,刘颖菊.龙胆苦苷对脓毒症所致急性肺损伤小鼠的保护作用[J].陆军军医大学学报(原第三军医大学学报),2012,34(23):2392-2394. DOI：

　Chen Lulu,Xu Guanli,Yuan Hongmei,Cai Jianghui,Liu Yingju.Protection of gentiopicroside on acute lung injury in sepsis mice[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(23):2392-2394. DOI：

摘要

目的观察龙胆苦苷对脓毒症急性肺损伤小鼠的保护作用。方法采用盲肠结扎穿孔的方法建立小鼠脓毒症急性肺损伤模型。80只18～22 g雄性昆明小鼠按随机区组设计分为假手术组、模型组、龙胆苦苷低剂量组(25 mg/kg)、龙胆苦苷高剂量组(50 mg/kg)，每组20只。各组于术前30 min尾静脉注射龙胆苦苷或生理盐水。术后12 h收集肺泡灌洗液检测其中炎症因子(TNF-α、IL-6)及总蛋白含量，测定肺组织中丙二醛(MDA)、髓过氧化物酶(MPO)、诱导型一氧化氮合酶(iNOS)、一氧化氮(NO)含量，光镜下观察肺组织病理改变情况。结果与模型组相比，龙胆苦苷低、高剂量组肺泡灌洗液中TNF-α、IL-6含量及总蛋白含量，肺组织中MDA、MPO、iNOS、NO水平均较低（P0.05，P0.01），且病理学损伤程度较模型组轻。结论龙胆苦苷能减轻炎症反应，对脓毒症所致急性肺损伤有保护作用。

Abstract

Objective To observe the protective effect of gentiopicroside on acute lung injury in sepsis mice. Methods The mouse model of septic acute lung injury was established by cecal ligation and puncture (CLP). Eighty 18-22 g male mice were randomly divided into a sham operation group (group A)

a model group (group B)

a low-dose gentiopicroside group (25 mg/kg

group C) and a high-dose gentiopicroside group (50 mg/kg

group D)

respectively (n=20). The mice were separately given a tail vein injection of gentiopicroside or normal saline at 30 min before CLP. At 12 h after operation

the contents of inflammatory factors (TNF-α and IL-6) and total protein in bronchoalveolar lavage fluid (BALF) were detected by ELISA

and the levels of malondialdehyde (MDA)

myeloperoxidase (MPO)

inducible nitric oxide synthase (iNOS) and NO in lung tissues were determined by biochemical methods. The histopathological changes of lung tissues were observed under a light microscope. Results Compared with group B

the contents of TNF-α

IL-6 and total protein in BALF and the levels of MDA

MPO

iNOS and NO in lung tissues decreased significantly in group C and group D (P0.05

P0.01)

and the pathological changes of lung tissues were attenuated by gentiopicroside. Conclusion Gentiopicroside

which can effectively attenuate inflammatory responses

plays a protective role in septic acute lung injury.

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Department of Anesthesiology, Department of War Wound Shock and Transfusion， Army Medical Center of PLA/Daping Hospital of Third Military Medical University

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