Role JAK2/STAT3 signaling pathway in invasion and vascular mimicry of liver cancer cells

庞林宾; 王洪林; 王晔飞; 李洪涛; 宋立文; 刘波

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Role JAK2/STAT3 signaling pathway in invasion and vascular mimicry of liver cancer cells

更新时间：2026-01-09

- Role JAK2/STAT3 signaling pathway in invasion and vascular mimicry of liver cancer cells
- Journal of Army Medical University Vol. 34, Issue 18, Pages: 1862-1865(2012)
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  CLC： R73-37;R730.23;R735.7
- Published：2012
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庞林宾,王洪林,王晔飞,李洪涛,宋立文,刘波.JAK2/STAT3通路在肝癌细胞侵袭及血管生成拟态中的作用[J].陆军军医大学学报(原第三军医大学学报),2012,34(18):1862-1865.

　Pang Linbin,Wang Honglin,Wang Yefei,Li Hongtao,Song Liwen,Liu Bo.Role JAK2/STAT3 signaling pathway in invasion and vascular mimicry of liver cancer cells[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(18):1862-1865.
庞林宾,王洪林,王晔飞,李洪涛,宋立文,刘波.JAK2/STAT3通路在肝癌细胞侵袭及血管生成拟态中的作用[J].陆军军医大学学报(原第三军医大学学报),2012,34(18):1862-1865. DOI：

　Pang Linbin,Wang Honglin,Wang Yefei,Li Hongtao,Song Liwen,Liu Bo.Role JAK2/STAT3 signaling pathway in invasion and vascular mimicry of liver cancer cells[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(18):1862-1865. DOI：

摘要

目的探讨JAK2/STAT3通路在肝癌细胞QGY-7701侵袭及血管生成拟态中的作用。方法 JAK2抑制剂AG490(5、10 μmol/L)处理肝癌细胞48 h，Transwell小室、体外成管实验分别检测各组细胞的体外侵袭能力和成管能力，RT-PCR检测Twist1及MMP-2 mRNA表达差异，Western blot检测STAT3、p-STAT3、Twist1和MMP-2蛋白表达差异。结果与对照组相比，Transwell小室穿膜细胞数减少，形成管道结构数目减少，Twist1及MMP-2 mRNA表达减少，Twist1、MMP-2和p-STAT3蛋白表达减少，差异均有统计学意义（P0.05），STAT3蛋白表达无差异（P0.05）。结论 AG490能有效抑制肝癌细胞侵袭及成管的能力，JAK2/STAT3通路在肝癌细胞侵袭及血管生成拟态中起促进作用。

Abstract

Objective To determine the role of JAK2/STAT3 signaling pathway in invasion and vascular mimicry of liver cancer cell line QGY-7701. Methods After QGY-7701 cells were treated with 5 and 10 μmol/L AG490 (the inhibitor of JAK2) for 48 h

the invasion and tube formation of tumor cells were tested with Transwell chamber test and vascular mimicry experiment. RT-PCR was used to measure the expression of Twist1 and MMP-2

and Western blot assay was employed to determine the expression of p-STAT3

STAT3

Twist1 and MMP-2. Results Compared to control group

there were less tumor cells permeating membrane and less formed tubes after AG490 treatment. RT-PCR showed that the expression of Twist1 and MMP-2 at mRNA level were decreased. Western blotting indicated that the expression of p-STAT3

Twist1

MMP-2 at protein levels were also decreased (P0.05)

while that of STAT3 protein had no difference among each group (P0.05). Conclusion AG490 significantly inhibits invasion and vascular mimicry of liver cancer cells in vitro

and JAK2/STAT3 signaling pathway promotes above processes.

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Related Institution

Department of Gastroenterology, Qingdao Central Hospital of Rehabilitation University

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Department of Stomatology， the First Affiliated Hospital of Shihezi University

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