Effects of MAPK or NF-κB pathway inhibitors on IL-1β-induced matrix metallo-proteinase-2 activity in synovial cells

陈荣富; 黄伟; 谢静; 梁熙; KL Paul Sung; 王春莉; 符纯锋

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Effects of MAPK or NF-κB pathway inhibitors on IL-1β-induced matrix metallo-proteinase-2 activity in synovial cells

更新时间：2026-01-09

- Effects of MAPK or NF-κB pathway inhibitors on IL-1β-induced matrix metallo-proteinase-2 activity in synovial cells
- Journal of Army Medical University Vol. 34, Issue 17, Pages: 1741-1744(2012)
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  CLC： R392.11;R392.3;R322.72
- Published：2012
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陈荣富,黄伟,谢静,梁熙,KL Paul Sung,王春莉,符纯锋.MAPK和NF-κB通路抑制剂对IL-1β诱导滑膜细胞MMP-2活性的影响[J].陆军军医大学学报(原第三军医大学学报),2012,34(17):1741-1744.

　Chen Rongfu,Huang Wei,Xie Jing,Liang Xi,KL Paul Sung,Wang Chunli,Fu Chunfeng.Effects of MAPK or NF-κB pathway inhibitors on IL-1β-induced matrix metallo-proteinase-2 activity in synovial cells[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(17):1741-1744.
陈荣富,黄伟,谢静,梁熙,KL Paul Sung,王春莉,符纯锋.MAPK和NF-κB通路抑制剂对IL-1β诱导滑膜细胞MMP-2活性的影响[J].陆军军医大学学报(原第三军医大学学报),2012,34(17):1741-1744. DOI：

　Chen Rongfu,Huang Wei,Xie Jing,Liang Xi,KL Paul Sung,Wang Chunli,Fu Chunfeng.Effects of MAPK or NF-κB pathway inhibitors on IL-1β-induced matrix metallo-proteinase-2 activity in synovial cells[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(17):1741-1744. DOI：

摘要

目的研究白介素-1β(Interleukin-1β

IL-1β)对人膝关节滑膜细胞基质金属蛋白酶-2(Matrix metalloproteinase-2

MMP-2)活性的影响及MAPK和NF-κB信号通路抑制剂对该过程的影响。方法用不同浓度的IL-1β处理体外培养的滑膜细胞72 h，明胶酶谱法检测细胞培养液中MMP-2的活性；选取10 ng/ml的IL-1β单独或分别与4种信号通路抑制剂(NF-κB抑制剂Bay11-7082，MAPK抑制剂SB203580、PD98059和SP600125)处理滑膜细胞，处理后12、24、48、72 h检测MMP-2的活性。结果 IL-1β随浓度和处理时间的增加明显上调滑膜细胞MMP-2的活性(P0.05)；与IL-1β处理组比较，Bay11-7082明显抑制了IL-1β诱导MMP-2活性的上调，并使72 h时MMP-2的活性降至13%(P0.01)；其他抑制剂对IL-1β诱导MMP-2活性上调的影响无统计学差异(P0.05)。结论 IL-1β以时间和剂量依赖方式，通过NF-κB信号通路诱导滑膜细胞MMP-2活性。

Abstract

Objective To study the effects of interlukin-1β (IL-1β) on the activity of matrix metalloproteinase-2 (MMP-2) in synovial cells from human knee joint

and the effects of MAPK or NF-κB signal pathway inhibitors on IL-1β-induced MMP-2 activity. Methods In vitro cultured synovial cells were treated with IL-1β at different concentrations

and gelatin zymography was performed to detect MMP-2 activity at 72 h. IL-1β of 10 ng/ml alone or in combination with each of four signal pathway inhibitors (NF-κB inhibitor: Bay11-7082; MAPK inhibitor: SB203580

PD98059 and SP600125) were used to treat the synovial cells

and MMP-2 activity was detected at 12

48 and 72 h

respectively. Results IL-1β significantly increased MMP-2 activity in synovial cells with increased concentration or time (P0.05). Bay11-7082 dramatically inhibited IL-1β-stimulated MMP-2 activity

and the activity was decreased to 13% compared with the IL-1β group (P0.01). However

other inhibitors had no significant effect on the IL-1β-stimulated MMP-2 activity(P0.05). Conclusion IL-1β significantly increases MMP-2 activity through NF-κB signal pathway in synovial cells in a dose- and time-dependent manner.

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