Objective        To investigate the effects of the recombinant adenovirus containing the triple-point mutants HIF1-α gene （Ad-HIF-1α-trip） on the expression of VEGF

the angiogenesis and the blood perfusion in the rat model of ischemic hind limb.       Methods       After ligation of left femoral artery

totally 48 male Wistar rats were randomly divided into 3 control groups (n=12 in each group) and model group (n=12). The control groups were subjected with normal saline (0.5 ml)

Ad-Null （4×10

9

PFU）or Ad-HIF-1α （4×10

9

PFU）respectively by intramuscular injection. The model group was subjected with Ad-HIF-1α-trip（4×10

9

PFU）. The expression of HIF-1α and VEGF at mRNA level in the ischemic skeletal muscle were detected by real-time PCR in 7 d after gene transfer. The changes of blood perfusion of the ischemic hind limb were detected by contrast enhanced ultrasonography (CEU) before operation

immediately and on the 7th

14th and 28th day after gene transfer. The microvascular density (MVD) of the ischemic hind limb were observed through immunohistochemistry of CD31.       Results       On the 7th day after gene transfer

the expression of HIF-1α and VEGF at mRNA level was more significant in the Ad-HIF-1α-trip group than other groups（HIF-1α: 2.576±0.019; VEGF: 2.498±0.031，P0.05）. CEU revealed better blood perfusion in Ad-HIF-1α-Trip group than in other groups (the standardized  value of A×β28 days after gene transfer: Saline vs Ad-Null vs Ad-HIF-1α vs Ad-HIF-1α-trip: 0.529±0.045 vs 0.535±0.062 vs 0.642±0.056 vs 0.895±0.049

P0.05). Immunohistochemical assay for CD31 showed the MVD of Ad-HIF-1α-trip was more denser than other groups in 28 d after gene transfer（Saline vs Ad-Null vs Ad-HIF-1α vs Ad-HIF-1α-trip: 82.316±19.258 vs 89.662±21.374 vs 175.248±31.736 vs 213.426±48.073

P0.05）.       Conclusion       Ad-HIF-1α-Trip is efficiently expressed in the ischemic skeletal muscle

and improves t

he angiogenesis and blood perfusion of the ischemic limb effectively.