Dendritic cells promote neural stem/progenitor cells differentiation through NT-3 up-regulating p-ERK1/2 in vitro

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Dendritic cells promote neural stem/progenitor cells differentiation through NT-3 up-regulating p-ERK1/2 in vitro

更新时间：2026-01-09

- Dendritic cells promote neural stem/progenitor cells differentiation through NT-3 up-regulating p-ERK1/2 in vitro
- Journal of Army Medical University Vol. 34, Issue 23, Pages: 2368-2372(2012)
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  CLC： R329.26;R329.28;R338.12
- Published：2012
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陈太邦,赵建华,王永飞,王钟,郭乔楠.体外树突状细胞通过NT-3上调P-ERK1/2的表达促进神经干/祖细胞分化[J].陆军军医大学学报(原第三军医大学学报),2012,34(23):2368-2372.

　Chen Taibang,Zhao Jianhua,Wang Yongfei,Wang Zhong,Guo Qiaonan.Dendritic cells promote neural stem/progenitor cells differentiation through NT-3 up-regulating p-ERK1/2 in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(23):2368-2372.
陈太邦,赵建华,王永飞,王钟,郭乔楠.体外树突状细胞通过NT-3上调P-ERK1/2的表达促进神经干/祖细胞分化[J].陆军军医大学学报(原第三军医大学学报),2012,34(23):2368-2372. DOI：

　Chen Taibang,Zhao Jianhua,Wang Yongfei,Wang Zhong,Guo Qiaonan.Dendritic cells promote neural stem/progenitor cells differentiation through NT-3 up-regulating p-ERK1/2 in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(23):2368-2372. DOI：

摘要

目的观察树突状细胞(dendritic cells

DCs)对神经干/祖细胞（neural stem/progenitor cells

NSPCs）分化的影响，探讨神经营养素-3（Neurotrophin-3，NT-3）在DCs调控NSPCs分化中可能的作用机制。方法实验共设5组，分别为NSPCs组、NSPCs/DCs组、NSPCs+NT-3组、NSPCs/DCs+抗NT-3组和DCs组。共培养24、48、72 h后，ELISA法检测各组上清液中NT-3含量；7 d后荧光免疫细胞化学法检测各组NSPCs分化情况。Western blot检测各组NSPCs磷酸化的细胞外调节蛋白激酶（extracellular regulated protein kinases

ERK1/2）表达。结果 NSPCs/DCs组上清液中NT-3含量较NSPCs组、NSPCs/DCs+抗NT-3组和DCs显著升高（P0.05）。NSPCs/DCs组和NSPCs+NT-3组β-tubulin-Ⅲ阳性细胞数较NSPCs组和NSPCs/DCs+抗NT-3组显著增高（P0.05），而NSPCs组和NSPCs/DCs+抗NT-3组GFAP阳性细胞较NSPCs/DCs组和NSPCs+NT-3组显著增高（P0.05）。NSPCs/DCs组和NSPCs+NT-3组NSPCs中的p-ERK1/2的表达较其余两组增高（P0.05）。结论 NSPCs与DCs共培养能显著促进NSPCs分化为神经元，可能与共培养后NT-3表达量增高，通过其特异性受体TrkC激活下游信号通路MEK-ERK有关。

Abstract

Objective To observe the effect of dendritic cells (DCs) on neural stem/progenitor cells (NSPCs) differentiation in vitro

and to explore the possible mechanism of neurotrophin-3 (NT-3) in NSPCs differentiation regulated by DCs. Methods Primary NSPCs and DCs generated from SD rats were separately cultured and then co-cultured by using transwell chambers

respectively. There are five experimental groups including a NSPCs group

a DCs group

a NSPCs/DCs group

a NSPCs+NT-3 group and a NSPCs/DCs+anti-NT-3 group. After incubation for 24

48 and 72 h

ELISA was used to detect the NT-3 content in cell supernatant of each group. After 7 d

fluorescence immunocytochemistry was used to detect NSPCs differentiation

and Western blot assay was used to detect the expression of phosphorylated extracellular signal-regulated protein kinases (p-ERK1/2) of NSPCs. Results The ELISA results showed that the NT-3 content in the cell supernatant of the NSPCs/DCs group was significantly increased compared with the NSPCs group

DCs group and NSPCs/DCs+anti-NT-3 group (P0.05). Seven days after induction of differentiation

the proportions of β-tubulin-Ⅲ-positive cells in the NSPCs/DCs group and NSPCs+NT-3 group were significantly higher than those of the NSPCs group and NSPCs/DCs+anti-NT-3 group (P0.05)

while the proportions of glial fibrillary acidic protein (GFAP)-positive cells in the NSPCs/DCs group and NSPCs+NT-3 group were significantly lower than those of the NSPCs group and NSPCs/DCs+anti-NT-3 group (P0.05). The Western blotting results showed that the levels of p-ERK1/2 in the NSPCs/DCs group and NSPCs+NT-3 group were significantly higher than those in the NSPCs group

DCs group and NSPCs/DCs+anti-NT-3 group (P0.05). Conclusion Co-culture of DCs and NSPCs can significantly promote NSPCs to differentiate into neurons. Activation of MEK-ERK signaling pathway may contribute to the differentiation of NSPCs into neurons

which is mediated by increased NT-3 binding to its specific receptor TrkC in co-culture system.

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