Effect of macrophage transplantation on extracellular matrix repair after myocardial infarction in rats

任宁; 周欣; 李贺; 黄体钢; 叶帆; 陈树涛; 丛洪良

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Effect of macrophage transplantation on extracellular matrix repair after myocardial infarction in rats

更新时间：2026-01-09

- Effect of macrophage transplantation on extracellular matrix repair after myocardial infarction in rats
- Journal of Army Medical University Vol. 34, Issue 17, Pages: 1727-1732(2012)
- 作者机构：
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  CLC： R329.25;R392.12;R542.22
- Published：2012
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任宁,周欣,李贺,黄体钢,叶帆,陈树涛,丛洪良.巨噬细胞移植对大鼠心肌梗死后细胞外基质修复的影响[J].陆军军医大学学报(原第三军医大学学报),2012,34(17):1727-1732.

　Ren Ning,Zhou Xin,Li He,Huang Tigang,Ye Fan,Chen Shutao,Cong Hongliang.Effect of macrophage transplantation on extracellular matrix repair after myocardial infarction in rats[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(17):1727-1732.
任宁,周欣,李贺,黄体钢,叶帆,陈树涛,丛洪良.巨噬细胞移植对大鼠心肌梗死后细胞外基质修复的影响[J].陆军军医大学学报(原第三军医大学学报),2012,34(17):1727-1732. DOI：

　Ren Ning,Zhou Xin,Li He,Huang Tigang,Ye Fan,Chen Shutao,Cong Hongliang.Effect of macrophage transplantation on extracellular matrix repair after myocardial infarction in rats[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(17):1727-1732. DOI：

摘要

目的探讨移植巨噬细胞对大鼠缺血再灌注引起的心肌梗死后细胞外基质修复和心室重塑的影响。方法贴壁法培养Wistar大鼠腹腔巨噬细胞，建立Wistar大鼠心肌缺血-再灌注模型，随机分为对照组（AMI组，n=23），巨噬细胞移植组（MΦ组，n=20），假手术组（n=12）。术后第7天和第28天，取大鼠左室心肌组织进行组织病理学分析，分别测定膨展指数、梗死面积、非梗死区心肌细胞横截面积（CSA）、梗死区及非梗死区胶原容积分数（CVF）、梗死区胶原成熟程度。结果与AMI组比较，MΦ组术后第7天膨展指数[（0.27±0.13）vs（0.41±0.19），P0.05

]

、非梗死区CSA[（330.65±57.30）μm

vs(408.00±99.98) μm

，P0.01

]

均显著减少，MΦ组术后第28天膨展指数[（0.30±0.11）vs(0.48±0.12，P0.01

]

、非梗死区CSA[（335.80±92.52） μm

vs (515.41±93.13) μm

，P0.01

]

也均显著减少；术后第7天和第28天MΦ组梗死区CVF显著大于AMI组[（53.51±5.82）% vs (45.05±3.66)%，(79.17±5.58)% vs (73.17±5.94)%，P0.01

]

，非梗死区CVF显著小于AMI组[（2.37±0.65）% vs (4.84±1.81)%，(2.64±0.63)% vs (6.01±1.34)%，P0.01

]

，梗死区胶原成熟程度显著大于AMI组[（0.566±0.009） vs (0.509±0.011)，(2.162±0.047) vs (1.454±0.020)，P0.01

]

。结论巨噬细胞心肌内移植促进梗死区胶原沉积和成熟度增加，减少梗死范围，减轻非梗死区纤维化程度和心肌肥厚程度，从而改善心肌梗死后左室重塑。

Abstract

Objective To investigate the effect of macrophages on extracellular matrix repair and ventricular remodeling after acute myocardial infarction (AMI) in rats induced by ischemia/reperfusion (I/R). Methods T

he peritoneal macrophages of Wistar rats were adherently cultured. The survival animals induced by I/R were randomly divided into a sham operation group (n=12)

an AMI group (n=23) and a macrophage transplantation group (MΦ group

n=20). At the 7th and 28th day after the induction of I/R

the histopathology of left ventricular myocardial tissues including index of expansion

infarct size

cardiomyocyte cross-sectional area (CSA) in non-infarct region

proportion of mature collagen fibers in infarct region and collagen volume fraction (CVF) in infarct region and non-infarct region were examined. Results Compared with the AMI group

the index of expansion and the CSA in non-infarct region at the 7th day (0.27±0.13 vs 0.41±0.19

P0.05; 330.65±57.30 vs 408.00±99.98 μm

P0.01) and the 28th day (0.30±0.11 vs 0.48±0.12

P0.01; 335.80±92.52 vs 515.41±93.13 μm

P0.01) all decreased in the MΦ group. The CVF in infarct region at the 7th day [(53.51±5.82)% vs (45.05±3.66)%

P0.01

]

and the 28th day [(79.17±5.58)% vs (73.17±5.94)%

P0.01

]

of the MΦ group were greater than those of the AMI group

while the CVF in non-infarct region at the 7th day [(2.37±0.65)% vs (4.84±1.81)%

P0.01

]

and the 28th day [(2.64±0.63)% vs (6.01±1.34)%

P0.01

]

of the MΦ group were less than those of the AMI group. Compared with the AMI group

the proportion of mature collagen fibers in infarct region increased in the MΦ group at the 7th day (0.566±0.009 vs 0.509±0.011

P0.01) and the 28th day (2.162±0.047 vs 1.454±0.020

P0.01). Conclusion Macrophage intra-myocardial transplantation can promote collagen deposition and maturation

reduce infarct size

and alleviate fibrosis and myocardial hypertrophy in non-infarct region

so as to improve left ventricular remodeling after AMI.

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Related Institution

Department of Laboratory Medicine， First Medical Center， Chinese PLA General Hospital

Graduate Institute， Chinese PLA Medical School

Department of Anesthesiology， Army Medical Center of PLA/Daping Hospital of Third Military Medical University

Department of Combat Trauma and Shock & Transfusion， Institute of Field Surgery， Army Medical Center of PLA/Daping Hospital of Third Military Medical University

Department of Pathophysiology， Key Laboratory of Extreme Environment Medicine of Ministry of Education， Faculty of High Altitude Military Medicine， Army Medical University （Third Military Medical University）

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