Mechanism of (-)-gossypol down-regulating B lymphocyte stimulator expression in Namalwa cells via autophagy

丁雯; 倪振洪; 程攀科; 王滨; 黄刚; 何凤田; 连继勤; 徐兴然

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Mechanism of (-)-gossypol down-regulating B lymphocyte stimulator expression in Namalwa cells via autophagy

更新时间：2026-01-09

- Mechanism of (-)-gossypol down-regulating B lymphocyte stimulator expression in Namalwa cells via autophagy
- Journal of Army Medical University Vol. 34, Issue 16, Pages: 1613-1616(2012)
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  CLC： R392.11;R967;R979.1
- Published：2012
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丁雯,倪振洪,程攀科,王滨,黄刚,何凤田,连继勤,徐兴然.左旋棉酚通过细胞自噬下调Namalwa细胞中B淋巴细胞刺激因子的表达[J].陆军军医大学学报(原第三军医大学学报),2012,34(16):1613-1616.

　Ding Wen,Ni Zhenhong,Cheng Panke,Wang Bin,Huang Gang,He Fengtian,Lian Jiqin,Xu Xingran.Mechanism of (-)-gossypol down-regulating B lymphocyte stimulator expression in Namalwa cells via autophagy[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(16):1613-1616.
丁雯,倪振洪,程攀科,王滨,黄刚,何凤田,连继勤,徐兴然.左旋棉酚通过细胞自噬下调Namalwa细胞中B淋巴细胞刺激因子的表达[J].陆军军医大学学报(原第三军医大学学报),2012,34(16):1613-1616. DOI：

　Ding Wen,Ni Zhenhong,Cheng Panke,Wang Bin,Huang Gang,He Fengtian,Lian Jiqin,Xu Xingran.Mechanism of (-)-gossypol down-regulating B lymphocyte stimulator expression in Namalwa cells via autophagy[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(16):1613-1616. DOI：

摘要

目的探讨左旋棉酚[(-)-gossypol]下调Namalwa细胞中B淋巴细胞刺激因子（B lymphocyte stimulator

BLyS）的可能机制。方法采用CCK8、台盼蓝活细胞染色检测左旋棉酚在体外对Namalwa细胞的生长抑制作用，吖啶橙（acridine orange， AO）染色观察经左旋棉酚处理后细胞自噬的形态特征，Western blot检测细胞中BLyS和自噬蛋白微管蛋白轻链3(LC3)的表达情况。结果左旋棉酚对Namalwa细胞有明显的增殖抑制作用，并且具有时间和剂量依赖性；AO染色后细胞内可观察到明显的细胞自噬小体；Western blot结果显示，左旋棉酚可下调Namalwa细胞中BLyS的表达，并上调LC3Ⅱ蛋白的表达；自噬特异性抑制剂3-methyladenine(3-MA)可显著抑制左旋棉酚对BLyS的下调与LC3Ⅱ的上调作用。结论左旋棉酚可能通过诱导细胞自噬来下调B淋巴细胞中BLyS的表达，从而抑制细胞生长。

Abstract

Objective To explore the mechanism of (-)-gossypol down-regulating B lymphocyte stimulator (BLyS) expression in human B-cell lymphoma cell line Namalwa. Methods The growth inhibition of Namalwa cells by (-)-gossypol was detected by CCK8 (cell counting kit-8) kit and trypan blue staining. Cell autophagy was analyzed by observing the autophagosome formation by acridine orange (AO) staining. The expression levels of BLyS and LC3 were detected by Western blotting. Results The growth of Namalwa cells was significantly inhibited by (-)-gossypol in a time- and dose-dependent manner. After the (-)-gossypol treatment

the amount of autophagosomes dramatically increased. The expression level of LC3 II was up-regulated and the expression level of BLyS was down-regulated in Namalwa cells after the treatment with (-)-gossypol

and this effect could be significantly attenuated by 3-methyladenine (3-MA). Conclusion (-)-gossypol may inhibit the growth of B lymphocytes through autophagy-medicated BLyS down-regulation.

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