Objective       It remains difficult to genotype the mixture of maternal and embryo from early abortion tissues to investigate forensic evidence and confirm parent-child relationship. Our study was aimed to confirm the parent-child relationship by using laser capture microdissection (LCM) to capture the embryonic villi cells in paraffin-embedded section for genotyping embryonic individual via ultralow volume PCR amplification.       Methods       Embryonic villi cells in the paraffin sections was captured by LCM technology. With the setting of cell numbers at 20

30 and 40 cells as a group

villus cells of different cell numbers were captured and subjected to PCR amplification at low volume of 1.5 μl by using 3130 Genetic Analysis instrument to yield embryonic genotyping maps. The artificial alleles

detection rate and allelic dropout rate of each group were compared. The technology was also applied on an actual case.       Results        Embryonic villi cells in the paraffin sections were captured by LCM technology. With the setting of cell numbers at 20

30 and 40 cells for group

villus cells of different cell numbers were captured and subjected to PCR amplification at low volume of 1.5 μl

and embryonic genotyping maps were obtained. The rate of embryo genotyping in the group of 40 cells was the highest whereas the rate of allele loss and non-specific amplification was the lowest. Vise versa for the group of 20 cells.       Conclusion        LCM combined with the low volume amplification technique can be applied to paraffin-embedded tissue sections to achieve accurate separation of the embryo and maternal components and obtain conclusive evidence for parental rights.