Alteration of reactive oxygen species in cytarabine-induced apoptosis process in MDS cell line MUTZ-8

罗静; 叶兴伟; 蒋文; 周洪静; 杨泽松; 刘林; 王利

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Alteration of reactive oxygen species in cytarabine-induced apoptosis process in MDS cell line MUTZ-8

更新时间：2026-01-09

- Alteration of reactive oxygen species in cytarabine-induced apoptosis process in MDS cell line MUTZ-8
- Journal of Army Medical University Vol. 34, Issue 15, Pages: 1492-1495(2012)
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  CLC： R73-361;R737.3;R979.12
- Published：2012
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罗静,叶兴伟,蒋文,周洪静,杨泽松,刘林,王利.阿糖胞苷诱导人MDS细胞株MUTZ-8细胞凋亡过程中活性氧水平的变化[J].陆军军医大学学报(原第三军医大学学报),2012,34(15):1492-1495.

　Luo Jing,Ye Xingwei,Jiang Wen,Zhou Hongjing,Yang Zesong,Liu Lin,Wang Li.Alteration of reactive oxygen species in cytarabine-induced apoptosis process in MDS cell line MUTZ-8[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(15):1492-1495.
罗静,叶兴伟,蒋文,周洪静,杨泽松,刘林,王利.阿糖胞苷诱导人MDS细胞株MUTZ-8细胞凋亡过程中活性氧水平的变化[J].陆军军医大学学报(原第三军医大学学报),2012,34(15):1492-1495. DOI：

　Luo Jing,Ye Xingwei,Jiang Wen,Zhou Hongjing,Yang Zesong,Liu Lin,Wang Li.Alteration of reactive oxygen species in cytarabine-induced apoptosis process in MDS cell line MUTZ-8[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(15):1492-1495. DOI：

摘要

目的探讨阿糖胞苷诱导人MDS细胞株MUTZ-8细胞凋亡的可能机制。方法将细胞分为以下5组：阴性对照组、0.1 μmol/L Ara-c组、1 μmol/L Ara-c组、10 μmol/L Ara-c组、10 μmol/L Ara-c+62.5 μmol/L ALA组。用MTT法测定阿糖胞苷对各组细胞抑制率的影响，用Annexin V-FITC/PI 双染法及Hoechst33258荧光染色观察阿糖胞苷对各组细胞凋亡的影响，并用流式细胞仪检测各组细胞线粒体膜电位的变化。运用二氢二氯荧光素(DCFH-DA)为细胞内活性氧探针，通过荧光显微镜及流式细胞仪检测各组细胞内活性氧水平的变化。结果 MTT结果提示，阿糖胞苷处理组细胞增殖抑制率高于阴性对照组，二者相比差异具有统计学意义（P0.05）。流式细胞仪结果提示，不同浓度的阿糖胞苷作用MUTZ-8细胞后，凋亡率明显升高，线粒体膜电位明显下降，与阴性对照组相比，差异具有统计学意义（P0.05）。流式细胞仪及荧光显微镜检测均显示，阿糖胞苷作用后细胞内活性氧（reactive oxygen species

ROS）明显上升。而加入抗氧化剂α-硫辛酸后细胞内ROS表达水平下降。结论阿糖胞苷明显抑制了人MDS细胞株MUTZ-8的生长并诱导其凋亡，可能与阿糖胞苷影响了细胞内ROS水平有关。

Abstract

Objective To investigate the possible underlying mechanism of apoptosis in MUTZ-8 cells induced by cytarabine. Methods The cells were divided into 5 groups

negative control group

0.1 μmol/L Ara-c group

1 μmol/L Ara-c group

10 μmol/L Ara-c group

and 10 μmol/L Ara-c+62.5 μmol/L ALA group. The proliferation of MUTZ-8 cells in above groups was measured by MTT assay. Apoptotic rate was assessed by Annexin V/PI double staining and luminescent dyes-Hoechst33258. Mitochondrial membrane potential of the cells was determined by flow cytometry. Using a ROS probe (2′

7′-dichlo-rofluorescein diacetate

DCFH-DA)

the level of reactive oxygen species (ROS) was detected by flow cytometry and fluorescence microscopy. Results MTT assay indicated cytarabine resulted in a stronger inhibition in cell viability when compared with the cells of negative control group (P0.05). Apoptotic rates of the cells in cytarabine groups were significantly increased as compared with negative control group (P0.05). Mitochondrial membrane potential levels of the cells were decreased in cytarabine groups as compared with negative control group (P0.05). ROS levels in all cytarabine groups were up-regulated as compared with negative control group (P0.05). Conclusion Cytarabine effectively inhibits the growth and induces the apoptosis in MUTZ-8 cells

which might be related to the elevation of ROS level.

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