Matrix metalloproteinase expression in posterior cruciate ligament fibroblasts co-cultured with synovial cells

王春莉; 梅虎; 谢静; 蒋稼欢; 尹琳; 陈诚; 符纯锋; KL Paul Sung

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Matrix metalloproteinase expression in posterior cruciate ligament fibroblasts co-cultured with synovial cells

更新时间：2026-01-09

- Matrix metalloproteinase expression in posterior cruciate ligament fibroblasts co-cultured with synovial cells
- Journal of Army Medical University Vol. 34, Issue 16, Pages: 1600-1603(2012)
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  CLC： R322.73;R329-33;R394.2
- Published：2012
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王春莉,梅虎,谢静,蒋稼欢,尹琳,陈诚,符纯锋,KL Paul Sung.共培养下后交叉韧带成纤维细胞中MMPs的基因表达[J].陆军军医大学学报(原第三军医大学学报),2012,34(16):1600-1603.

　Wang Chunli,Mei Hu,Xie Jing,Jiang Jiahuan,Yin Lin,Chen Cheng,Fu Chunfeng,KL Paul Sung.Matrix metalloproteinase expression in posterior cruciate ligament fibroblasts co-cultured with synovial cells[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(16):1600-1603.
王春莉,梅虎,谢静,蒋稼欢,尹琳,陈诚,符纯锋,KL Paul Sung.共培养下后交叉韧带成纤维细胞中MMPs的基因表达[J].陆军军医大学学报(原第三军医大学学报),2012,34(16):1600-1603. DOI：

　Wang Chunli,Mei Hu,Xie Jing,Jiang Jiahuan,Yin Lin,Chen Cheng,Fu Chunfeng,KL Paul Sung.Matrix metalloproteinase expression in posterior cruciate ligament fibroblasts co-cultured with synovial cells[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(16):1600-1603. DOI：

摘要

目的研究后交叉韧带（posterior cruciate ligament

PCL）成纤维细胞与滑膜细胞共培养下，2种细胞之间的交流对PCL成纤维细胞中基质金属蛋白酶MMP-1、2、3表达及活性的影响。方法分为共培养组和单培养组：①用显微镜观察共培养24 h后细胞的活性和迁移率。②分别培养6 h后，提取总RNA，逆转录PCR；实时荧光定量PCR对人后交叉韧带成纤维细胞MMP-1、2、3基因的表达进行半定量和定量分析。③共培养处理1、2、3 d后收集培养上清液，用明胶酶谱法检测MMP-2的活性。结果共培养使PCL细胞和滑膜细胞较单层细胞培养迁移率分别增高了（43.1±0.1）%和（76.2±0.2）%(P0.01)。与单培养相比，共培养组明显增加了MMP-2的基因表达，但降低了MMP-1、3的基因表达。酶谱结果表明共培养增加了MMP-2活性（P0.05）。结论共培养能够促进细胞的迁移以及调节细胞中MMP-1、2、3的表达情况。

Abstract

Objective To investigate the expression of matrix metalloproteinase (MMP)-1

MMP-2 and MMP-3 in posterior cruciate ligament (PCL) fibroblasts co-cultured with synovial cells. Methods Human PCL fibroblasts were co-cultured with synovial cells and the monocultures of human PCL fibroblasts and synovial cells were assigned as control. Cell viability and migration were observed by microscopy after 24 h incubation. Total RNA was isolated after 6 h incubation and the expression of MMPs was analyzed by semi-quantitative PCR and quantitative real-time PCR. Gelatin zymography was applied to detect the MMP-2 activity in culture supernatants collected at 1

2 and 3 d after incubation. Results As compared with the monocultures

the migration rates of the human PCL fibroblasts and synovial cells in the co-culture increased by (43.1±0.1)% and (76.2±0.2)%

respectively (P0.01). The mRNA expression levels of MMP-1 and MMP-3 in the PCL fibroblasts co-cultured with synovial cells were lower than those in the PCL fibroblasts monoculture. However

PCL fibroblasts-synovial cells interaction significantly increased the expression and activity of MMP-2 in the PCL fibroblasts (P0.05). Conclusion The differential expression of MMP-1

MMP-2 and MMP-3 in human PCL fibroblasts co-cultured with synovial cells indicates that cell-cell interaction and cross-talking are associated with PCL fibroblasts wound healing and have potential clinical value for curing injured PCL.

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