Tauroursodeoxycholic acid blocks lipolysis induced by TNF-α in 3T3-L1 adipocytes

夏文燕; 王丽静; 刘小莺; 林益川; 刘礼斌

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Tauroursodeoxycholic acid blocks lipolysis induced by TNF-α in 3T3-L1 adipocytes

更新时间：2026-01-09

- Tauroursodeoxycholic acid blocks lipolysis induced by TNF-α in 3T3-L1 adipocytes
- Journal of Army Medical University Vol. 34, Issue 12, Pages: 1206-1209(2012)
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  CLC： R341;R589.2;R963
- Published：2012
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夏文燕,王丽静,刘小莺,林益川,刘礼斌.牛黄熊脱氧胆酸抑制TNF-α刺激3T3-L1细胞脂肪分解[J].陆军军医大学学报(原第三军医大学学报),2012,34(12):1206-1209.

　Xia Wenyan,Wang Lijing,Liu Xiaoying,Lin Yichuan,Liu Libin.Tauroursodeoxycholic acid blocks lipolysis induced by TNF-α in 3T3-L1 adipocytes[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(12):1206-1209.
夏文燕,王丽静,刘小莺,林益川,刘礼斌.牛黄熊脱氧胆酸抑制TNF-α刺激3T3-L1细胞脂肪分解[J].陆军军医大学学报(原第三军医大学学报),2012,34(12):1206-1209. DOI：

　Xia Wenyan,Wang Lijing,Liu Xiaoying,Lin Yichuan,Liu Libin.Tauroursodeoxycholic acid blocks lipolysis induced by TNF-α in 3T3-L1 adipocytes[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(12):1206-1209. DOI：

摘要

目的探讨牛黄熊脱氧胆酸（tauroursodeoxycholic acid ，TUDCA）对TNF-α刺激3T3-L1细胞脂肪分解及其可能的机制。方法以3T3-L1前脂肪细胞经1-甲基-3-异丁基-黄嘌呤、地塞米松、胰岛素诱导分化成3T3-L1脂肪细胞，设：①对照组；②TNF-α（50 ng/ml）组；③TUDCA（1 mmol/L）+TNF-α（50 ng/ml）组；④TUDCA组（1 mmol/L）。通过油红O染色观察脂滴形态变化并测定培养基中甘油含量作为评价脂肪分解的指标，同时用Western blot 检测脂滴包被蛋白perilipin A蛋白表达。结果 TNF-α可以显著刺激3T3-L1细胞脂肪分解，包括功能学显示甘油释放量显著增加[(2.784±0.104)mmol/L，P0.01]，形态学显示脂滴碎裂；TUDCA可以明显抑制TNF-α刺激的3T3-L1细胞脂肪分解，包括阻止脂滴形态的改变，逆转甘油的释放[(1.718±0.085)mmol/L，P0.01]。通过Western blot 检测结果显示TNF-α可以下调perilipin A蛋白表达[（0.227±0.004），P0.01]，而TUDCA可以阻断TNF-α对perilipin A蛋白表达的下调作用[（0.705±0.066），P0.01]。结论 TUDCA可能通过阻止perilipin A蛋白下调而抑制TNF-α诱发脂肪分解，通过减少游离脂肪酸FFA，从而对胰岛素抵抗、糖尿病等具有一定的改善作用。

Abstract

Objective To explore the effect of tauroursodeoxycholic acid (TUDCA) on TNF-α-induced lipolysis in 3T3-L1 adipocytes. Methods The 3T3-L1 adipocytes obtained from 3T3-L1 preadipocytes induced by the cocktail medium containing 3-isobutyl-1-methylxanthine(IBMX)

dexamethasone and insulin were divided into 4 groups

including a control group

a TNF-α (50 ng/ml) group

a TUDCA (1 mmol/L) group and a TNF-α (50 ng/ml)+TUDCA (1 mmol/L) group. The intracellular lipid droplets were stained by oil red O. The content of glycerol released in medium was determined by colorimetric assay. The expression of perilipin A was detected by Western blotting. Results TNF-α significantly induced lipolysis in 3T3-L1 adipocytes. The content of glycerol released in medium significantly increased (2.784±0.104 mmol/L

P0.01)

and the lipid droplets dispersed. TUDCA could significantly block the lipolysis induced by TNF-α by inhibiting the dispersion of lipid droplets and reversing the glycerol release (1.718±0.085 mmol/L

P0.01). The Western blotting showed that TNF-α could downregulate the expression of perilipin A (0.227±0.004

P0.01)

which could be blocked by TUDCA (0.705±0.066

P0.01). Conclusion TUDCA attenuates TNF-α-induced lipolysis by blocking the downregulation of perilipin A

and reduces the concentration of free fatty acids to improve insulin resistance and type 2 diabetes.

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