Objective      To establish a preliminary dog animal models of silicosis in order to simulate silicosis disease process.       Methods      After making the silica particles blowing in the lung of the dog

tracheal intubation and breathing machine ventilation

we observed in different doses

different frequencies

different durations

the modifications of experimental dogs of arterial blood gas

Chest CT imaging

lung tissue pathological morphology.       Results      There was no significant difference in the arterial blood gas levels of experimental dogs (P0.05)

but the chest CT imaging had different degrees of ground glass samples change after dyed silica. Lung tissue pathology showed the interstitial lung thickening

many swallowed dust macrophages gathered around the final fine bronchial wall

fibroblasts hyperplasia

and mesh collagen fibers formation. Polarized light microscopy displayed lots of silica particles deposit in the interstitial lung.       Conclusion      Use of tracheal intubation and breathing machine ventilation

weigh silica particles according to 400 mg/kg and make them blowing in the lung of the dog

once a week for three months

we can establish a preliminary dog animal models of silicosis.