Objective      To construct the aptamer-based surface plasma resonance (SPR) microarray for rapid assay of toxoplasma gondii (TOX) and cytomegalovirus (CMV) IgG antibodies.       Methods      Aptamers of the TOX gondii and CMV IgG antibodies were screened with the SELEX technique and integrated into the real-time online analyzing system on the SPR biosensor. Hybridization of TOX and CMV IgG antibodies in solution was detected according to the probe molecules fixed on the surface of biosensor. The stability and linear detection range of this assay were further investigated.       Results      The novel rapid method could assay the TOX and CMV IgG antibodies with a good stability and a linear assay range of 20 to 300 μmol/L.       Conclusion      The stability of the rapid assay we established is good. Combined SPR biosensor and aptamer techniques have a broad prospect in clinical assay of TOX and CMV IgG antibody level.